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1.14.14.36: tyrosine N-monooxygenase

This is an abbreviated version!
For detailed information about tyrosine N-monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.36

Reaction

L-tyrosine
+ 2 O2 + 2 [reduced NADPH-hemoprotein reductase] =
(E)-[4-hydroxyphenylacetaldehyde oxime]
+ 2 [oxidized NADPH-hemoprotein reductase] +
CO2
+ 3 H2O

Synonyms

CYP79, CYP79A1, cytochrome P-450tyr, cytochrome P450 CYP79E1, cytochrome P450 CYP79E2, Cytochrome P450Tyr, EC 1.14.13.41, P450Tyr, tyrosine N-hydroxylase, Tyrosine N-monooxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.36 tyrosine N-monooxygenase

Purification

Purification on EC 1.14.14.36 - tyrosine N-monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
combined use of Renex 690, CHAPS and RTX-100 is optimal for maximal recovery and avoidance of conversion into cytochrome P-420
-
DEAE-Speharose column chromatography and Reactive Red-120 agarose column chromatography
-
homogeneity
-