Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.14.3: bacterial luciferase

This is an abbreviated version!
For detailed information about bacterial luciferase, go to the full flat file.

Word Map on EC 1.14.14.3

Reaction

a long-chain aldehyde
+
FMNH2
 +
O2
=
a long-chain fatty acid
 +
FMN
 +
H2O
 +
hnu

Synonyms

4a-hydroperoxy-4a,5-dihydroFMN intermediate luciferase, aldehyde monooxygenase, alkanal monooxygenase (FMN), bacterial luciferase, COB, Gluc luciferase, HFOOH, luciferase, Lux, LuxA, LuxAB, LuxB, LuxCDABE, LuxF, Vibrio fischeri luciferase, Vibrio harveyi luciferase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.3 bacterial luciferase

Inhibitors

Inhibitors on EC 1.14.14.3 - bacterial luciferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,4-benzoquinone
-
-
1-aminodecanal
-
-
1-aminododecanal
-
-
1-Decanol
-
-
1-Dodecanol
1-Heptanol
-
-
1-nonanol
-
-
1-Octanol
-
-
1-Tetradecanol
-
-
1-undecanol
-
-
2,2-diphenylpropylamine
-
-
2,3-Dichloro-(6-phenylphenoxy)ethylamine
2,4-dinitrofluorobenzene
-
i.e. Sanger's reagent
2-Bromodecanal
-
protection by dithiothreitol or mercaptoethanol
2-diethylaminoethyl-2,2-diphenylvalerate
2-methyl-1,4-benzoquinone
-
-
2-methyl-5-isopropyl-1,4-benzoquinone
-
-
5-decyl-4a-hydroxy-4a,5-dihydroriboflavin-5'-phosphate
-
binding and fluorescence quantum yield studies of the substance as a model, complexed with the enzyme in a 1:1 molcular ratio leading to 80% and 90% inhibition of wild-type and mutant C106A at 0.01 mM, respectively, binds to the active site
6-(3'-(R)-myristyl) flavin adenine mononucleotide
binds noncovalently to the enzyme dimer. Addition of recombinant apo-LuxF captures myrFMN and thereby relieves the inhibitory effect
8-Anilino-1-naphthalenesulfonate
acetone
-
-
aliphatic alcohols
-
-
Aliphatic alkanes
-
-
-
amino group reagents
-
benzylalcohol
-
-
Butanone
-
-
chloroform
-
-
Decanoic acid
-
-
diethylether
-
-
dodecanamide
-
inhibits bacterial luciferase luminescence reaction. By injecting the dodecaneamide into the bacterial luciferase system, the luminescence intensity decreases to about half of the initial intensity
dodecanenitrile
-
-
dodecanoic acid
-
-
Dodecanol
-
-
dodecylamine
-
-
Enflurane
-
-
ethoxyformic anhydride
Fluroxene
-
-
fullerenol
-
fullerenols suppress bioluminescent intensity at concentrations above 0.01 g/l and above 0.001 g/l for C60O2-4(OH)20-24 and Fe0.5C60(OH)xOy, respectively
Halothane
-
-
imidazole reagents
-
iodoacetamide
-
-
Isoflurane
-
-
methanol
-
bacterial luciferase luminescence intensity decreases to the steady state depending on the methanol concentration
Methoxyflurane
-
-
N,N-Diethyl-2,4-dichloro-(6-phenylphenoxy)ethylamine
-
-
N,N-Dimethylaniline
-
-
n-decanal
N-ethylmaleimide
-
protection by substrates
N-phenacylthiazolium bromide
-
highly selective inhibition
Paraldehyde
-
-
Pargyline
-
-
pifithrin-alpha
-
highly selective inhibitor in vivo and in vitro
potassium iodide
-
quenches the fluorescence of FMN effectively at 0.2 M, and enhances the decay of wild-type and HFOOH enzymes, the wild-type enzyme forms an inactive complex with KI
Proteases
-
trypsin, chymotrypsin
-
reduced riboflavin
-
-
SKF-525A
-
-
sulfhydryl reagents
trans-2-decenal
-
-
Tridecanoic acid
-
-
Undecanal
-
-
undecane
-
-
Urea
-
denaturation curve, thermodynamics, wild-type and mutants, overview
urethane
-
-
additional information
-