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1.14.14.22: dibenzothiophene sulfone monooxygenase

This is an abbreviated version!
For detailed information about dibenzothiophene sulfone monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.22

Reaction

dibenzothiophene-5,5-dioxide
+
FMNH2
 +
NADH
 +
O2
=
2'-hydroxybiphenyl-2-sulfinate
 +
H2O
 +
FMN
 +
NAD+
 +
H+

Synonyms

BdsA, DBT sulfone monooxygenase, DBTO2-MO, DBTO2-monooxygenase, dibenzothiophene sulfone monooxygenase, dszA, TdsA

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.22 dibenzothiophene sulfone monooxygenase

Cloned

Cloned on EC 1.14.14.22 - dibenzothiophene sulfone monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene bdsA, recombinant overexpression in Escherichia coli, coexpression with chaperone groEL/groES genes
-
gene dszA
gene dszA, cloning from dszABC operon, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis
gene dszA, DNA and amino acid sequence determination and analysis
-
gene dszA, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha
-
gene dszA, DNA and amino acid sequence determination and analysis, sequence comparisons
gene dszA, recombinant expression of the complete dszABC operon, revealing that the ratio of mRNA quantity of dszA, dszB, and dszC is 11:3.3:1, the expression level of dszB is far lower than that of dszC. The termination codon of dszA and the initiation codon of dszB overlap, whereas there is a 13-bp gap between genes dszB and dszC. Quantitative analysis of dsz operon transcription by real-time PCR
gene dszA, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain JM109
-
gene tdsA, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression in Escherichia coli, coexpression with genes tdsC and tdsB, leading to established thermophilic desulfurization of dibenzothiophene in Escherichia coli host, an Escherichia coli oxidoreductase can be functionally coupled with the monooxygenases of a gram-positive thermophile. The end of tdsA shows a 4-bp overlap with the translational start site of tdsB