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1.14.14.20: phenol 2-monooxygenase (FADH2)

This is an abbreviated version!
For detailed information about phenol 2-monooxygenase (FADH2), go to the full flat file.

Word Map on EC 1.14.14.20

Reaction

phenol
+
FADH2
 +
O2
=
catechol
 +
FAD
 +
H2O

Synonyms

FAD reductase, flavin reductase PheA2, PheA, PheA1, PheA2, phenol hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.20 phenol 2-monooxygenase (FADH2)

Cloned

Cloned on EC 1.14.14.20 - phenol 2-monooxygenase (FADH2)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene pheA1, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli, cloning of the pheA1 promoter from Geobacillus thermoglucosidasius and use for enzyme expression in Escherichia coli strain Rosetta, tandem expression of genes pheA1 and pheA2
gene pheA1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli, coexpression with pheA2 is required for catalytic activity
gene pheA1, quantitative real-time PCR-based expression and promoter activity analysis, recombinant expression in Corynebacterium glutamicum strain RES167, a heterologous host lacking the PheR regulator, coexpression of gene pheA1 with gene pheA2 and regulator gene pheR, subcloning in Escherichia coli strain DH5alpha
gene pheA1, recombinant expression in Escherichia coli, tandem expression with pheA2 does not result in PheA2 protein
gene pheA2, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain Rosetta tandem expression of genes pheA1 and pheA2
gene pheA2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli, coexpression with pheA1 is required for catalytic activity
gene pheA2, recombinant expression in Escherichia coli, tandem expression with pheA1 does not result in PheA2 protein
gene pheA2, sequence comparisons, recombinant overexpression of wild-type and selenomethionine-substituted PheA2 in Escherichia coli strain BL21(DE3)pLysS
-
genes pheA1(1-3), phylogenetic analysis, typical for genes of the peripheral degradation of aromatic compounds, pheA1(1–3) and pheA2(1-3) are not located within gene clusters for central ortho- and meta-cleavage pathway. All three gene sets are nearby to genes with function in (chloro)aromatic degradation
PheA1 and PheA2 are separately expressed in recombinant Escherichia coli BL21 pLysS cells
phenol hydroxylase is a two-component flavin-dependent monooxygenase, the two proteins are encoded by the genes pheA1 and pheA2, located very closely in the genome, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged PheA1 in Escherichia coli strain M15 by nickel affinity chromatography
phenol hydroxylase is a two-component flavin-dependent monooxygenase, the two proteins are encoded by the genes pheA1 and pheA2, located very closely in the genome, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged PheA2 in Escherichia coli strain M15
plasmid pQE30A2 expressing His6PheA2 protein transformed into Escherichia coli M15
plasmid pQE9A1 expressing His6PheA1 protein transformed into Escherichia coli M15