1.14.14.18: heme oxygenase (biliverdin-producing)
This is an abbreviated version!
For detailed information about heme oxygenase (biliverdin-producing), go to the full flat file.
Word Map on EC 1.14.14.18
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1.14.14.18
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monoxide
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cytoprotective
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endothelial
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necrosis
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dismutase
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protoporphyrin
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erythroid
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2-related
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tnf
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bilirubin
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artery
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malondialdehyde
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lipopolysaccharide
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catalase
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hemin
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neuroprotective
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ischemia
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gsh
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lps
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sod
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anti-oxidant
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mapks
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sirna
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caspase-3
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quinone
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pulmonary
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reperfusion
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lps-induced
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hypoxia
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cyclooxygenase-2
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cox-2
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ferritin
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erk
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nf-e2-related
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anti-apoptotic
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cobalt
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myeloperoxidase
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ischemia-reperfusion
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nrf2-mediated
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pro-oxidant
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kelch-like
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nadph:quinone
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factor-2
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lps-stimulated
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2-like
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erythroid-derived
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oxygenases
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delta-aminolevulinic
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sulforaphane
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hyperbilirubinemia
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medicine
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drug development
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analysis
- 1.14.14.18
- monoxide
-
cytoprotective
- endothelial
- necrosis
- dismutase
- protoporphyrin
-
erythroid
-
2-related
- tnf
- bilirubin
- artery
- malondialdehyde
- lipopolysaccharide
- catalase
- hemin
-
neuroprotective
- ischemia
- gsh
- lps
- sod
-
anti-oxidant
- mapks
- sirna
- caspase-3
- quinone
- pulmonary
-
reperfusion
-
lps-induced
- hypoxia
- cyclooxygenase-2
- cox-2
- ferritin
- erk
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nf-e2-related
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anti-apoptotic
- cobalt
- myeloperoxidase
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ischemia-reperfusion
-
nrf2-mediated
-
pro-oxidant
-
kelch-like
-
nadph:quinone
-
factor-2
-
lps-stimulated
-
2-like
-
erythroid-derived
- oxygenases
-
delta-aminolevulinic
- sulforaphane
- hyperbilirubinemia
- medicine
- drug development
- analysis
Reaction
+ 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = + + + 3 [oxidized NADPH-hemoprotein reductase] + 3 H2O
Synonyms
biliverdin-producing heme oxygenase, ChuS, ChuZ, EC 1.14.99.3, haem oxygenase, heme oxygenase, heme oxygenase 1, heme oxygenase 2, heme oxygenase-1, heme oxygenase-2, HemO, Hmox1, Hmox1a, Hmox1b, Hmox2, Hmox2a, Hmox2b, HmuO, Hmx1, HO, HO-1, HO-2, Ho1, Ho2, Ho3, HO4, HSP32, HugZ, HY1, inducible heme oxygenase-1, More, MsHO1, ORP33 proteins, oxygenase, heme (decyclizing), pbsA1, PigA, proteins, specific or class, ORP33 (oxygen-regulated protein 33,000-mol.-wt.), Syn HO-1, Syn HO-2
ECTree
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Purification
Purification on EC 1.14.14.18 - heme oxygenase (biliverdin-producing)
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2 separate fractions after first DEAE-cellulose, hydroxyapatite, Sephadex G-150, DEAE-cellulose
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ammonium sulfate, Sephadex G-75, DEAE-cellulose, hydroxyapatite, tryptic 28000 Da fragment of recombinant heme oxygenase-2
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DEAE-ion exchange expanded bed adsorption and Superdex 75 gel filtration, the specific HO-1 activity increases from 0.82 to 24.8 U/mg during purification steps
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deletion of 23 C-terminal amino acids, which serve as the membrane-spanning domain, allows for a rapid purification of truncated, soluble HO-1 in large quantities, by standard column chromatography
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HY1, HO3, and HO4. HO3 and HO4 purified by cobalt affinity chromatography, to over 90% purity
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hydroxylapatite column chromatography and POROS-HQ column chromatography
on Ni-NTA column, wild-type 32fold purified and mutant H25A 31fold purified, more than 95% pure
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partial
recombinant C-terminal truncated heme oxygenase-1, ammonium sulfate, Mono Q, recombinant heme oxygenase-1/cytochrome P450 reductase fusion protein, ammonium sulfate, 2',5'-ADP-Sepharose
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recombinant ChuZ from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
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recombinant GST-tagged full-length HO-1 mutant R254K 54fold and recombinant wild-type enzyme 40fold by thrombin treatment and glutathione affinity chromatography, elution with 1.0% sarkosyl detergent and 2% octyl glucoside, the recombinant wild-type full-length enzyme is not stable during expression and purification due to proteolytic cleavage, overview
recombinant GST-tagged wild-type and mutant HO-2 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
recombinant His-tagged HO1 from Escherichia coli by nickel affinity chromatography
recombinant His6-tagged HugZ from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
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recombinant wild-type and His-tagged enzyme, ion-exchange, gel filtration
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Triton X-100 + cholate, DEAE-cellulose, hydroxyapatite, Sepharose CL-6B, hydroxyapatite
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verdoheme-HmuO complex by anion exchange chromatography and gel filtration