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1.14.14.11: styrene monooxygenase

This is an abbreviated version!
For detailed information about styrene monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.11

Reaction

Styrene
+
FADH2
+
O2
=
(S)-2-phenyloxirane
+
FAD
+
H2O

Synonyms

FAD-specific styrene epoxidase, NSMOA, SMO, SmoA, StyA, StyA1, StyA1/StyA2B, StyA2B, StyAB

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.11 styrene monooxygenase

Purification

Purification on EC 1.14.14.11 - styrene monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
N-terminally histidine-tagged styrene monooxygenase
Ni-NTA affinity column chromatography
-
Ni-Sepharose column chromatography and MonoQ column chromatography
nickel-affinity column chromatography
-
Toyopearl SuperQ-650 M column chromatography, Ni-Sepharose column chromatography, MonoQ column chromatography and Superdex 200 gel filtration