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1.14.14.11: styrene monooxygenase

This is an abbreviated version!
For detailed information about styrene monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.11

Reaction

Styrene
+
FADH2
 +
O2
=
(S)-2-phenyloxirane
 +
FAD
 +
H2O

Synonyms

FAD-specific styrene epoxidase, NSMOA, SMO, SmoA, StyA, StyA1, StyA1/StyA2B, StyA2B, StyAB

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.11 styrene monooxygenase

Engineering

Engineering on EC 1.14.14.11 - styrene monooxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
L44A
mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene
L45A
mutation leads to a change of substrate preference. The mutant enzyme exhibits a remarkably reversed substrate preference compared to the wild type, displaying increased reactivity with an increase in substrate size. The activity of L45A is about 5% of that of the wild type towards styrene, but reaches about 30 and about 90% when the substrate is changed to 2-methylstyrene and 2-ethylstyrene, respectively
N46A
mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene
R43A
mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene
L44A
-
mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene
-
L45A
-
mutation leads to a change of substrate preference. The mutant enzyme exhibits a remarkably reversed substrate preference compared to the wild type, displaying increased reactivity with an increase in substrate size. The activity of L45A is about 5% of that of the wild type towards styrene, but reaches about 30 and about 90% when the substrate is changed to 2-methylstyrene and 2-ethylstyrene, respectively
-
N46A
-
mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene
-
R43A
-
mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene
-
T200V
mutant exhibits lower reactivity than the wild type, displayed impaired diastereoselectivity
Y73F
mutant exhibits lower reactivity than the wild type
additional information