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1.14.13.B28: monooxygenase CYP119A2

This is an abbreviated version!
For detailed information about monooxygenase CYP119A2, go to the full flat file.

Word Map on EC 1.14.13.B28

Reaction

Styrene
+
NADH
 +
H+
 +
O2
=
styrene epoxide
 +
NAD+
 +
H2O

Synonyms

CYP119, cytochrome P450 119, P450st

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.B28 monooxygenase CYP119A2

Crystallization

Crystallization on EC 1.14.13.B28 - monooxygenase CYP119A2

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
molecular modeling of structure
-
vapor diffusion method using a protein solution (15 mg/ml) and a reservoir solution (30% (w/v) polyethylene glycol 4000, 200 mM lithium sulfate monohydrate and 100 mM Tris-HCl, pH 8.5)
vapour diffusion method, X-ray crystallography at a resolution of 1.94 A reveals a sufficiently large heme pocket for NAD(P)H binding and a novel contiguous channel from the active site to bulk solvent in the distal heme pocket. The mutant shows a higher affinity for NADH compared with the wild-type because the mutant has a more widely open distal pocket for NAD(P)H binding