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1.14.13.82: vanillate monooxygenase

This is an abbreviated version!
For detailed information about vanillate monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.82

Reaction

vanillate
+
O2
+
NADH
+
H+
=
3,4-dihydroxybenzoate
+
NAD+
+
H2O
+
formaldehyde

Synonyms

4-hydroxy-3-methoxybenzoate demethylase, EC 1.2.3.12, IvaAB, More, VAN, VanA, vanAB, VanB, vanillate demethylase, vanillate O-demethylase, vanillate O-demethylase oxygenase, vanillate-O-demethylase, vanillic acid O-demethylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.82 vanillate monooxygenase

Cloned

Cloned on EC 1.14.13.82 - vanillate monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
-
expression in Escherichia coli
-
gene ivaA, DNA and amino acid sequence determination and analysis, genetic organization, physical map, expression analysis, recombinant expression in Escherichia coli
gene ivaB, DNA and amino acid sequence determination and analysis, genetic organization, physical map, expression analysis, recombinant expression in Escherichia coli
gene vanA, DNA and amino acid sequence determination and analysis, genetic organization, physical map, expression analysis, recombinant expression in Escherichia coli
gene vanAB, recombinant overexpression in Corynebacterium glutamicum strain F, and the strain produces vanillate from ferulic acid and the enzyme converts it to protocatechiuc acid, a precursor of polymers and plastics and a constituent of food, fed-batch biotransformation
Q8FRX4; Q8FRX3
genes vanA and vanB, DNA and amino acid sequence determination and analysis, functional coexpression of genes vanA and vanB in Escherichia coli K12 strain WV1181
-
genes vanA and vanB, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparison, functional coexpression of genes vanA and vanB in Streptomyces lividans strain 1326 from the high-copy plasmid pIJ702, selection on veratric acid
-
genes vanAB, functional expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha, VanA is expressed as a soluble protein, whereas VanB is expressed in inclusion bodies
Q08KC3; Q08KC3