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1.14.13.81: magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase

This is an abbreviated version!
For detailed information about magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase, go to the full flat file.

Word Map on EC 1.14.13.81

Reaction

131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester
+
NADPH
 +
H+
 +
O2
=
131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester
 +
NADP+
 + 2 H2O

Synonyms

AcsF, AcsF protein, aerobic Mg-protoporphyrin IX monomethyl ester cyclase, ATZIP protein, BChE, BNZIP protein, CHL27, CHL27 protein, CRD1, Crd1 protein, CSZIP protein, Cth1 protein, CycI, cyclase, magnesium protoporphyrin IX monomethyl ester, LCAA, LOC_Os01g17170, magnesium protoporphyrin IX monomethyl ester cyclase, magnesium protoporphyrin monomethylester oxidative cyclase, magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase, magnesium-protoporphyrin IX monomethyl ester cyclase, magnesium-protoporphyrin-IX monomethyl ester cyclase, magnesium-protoporphyrin-IX monomethyl ester oxidative cyclase, Mg protoporphyrin monomethylester cyclase, Mg-protoporphyrin IX methyl ester cyclase, Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase, Mg-protoporphyrin IX monomethyl ester cyclase, Mg-protoporphyrin IX monomethylester cyclase system, Mg-protoporphyrin monomethylester cyclase, MgPME cyclase, MgPME oxidative cyclase, MPE cyclase, MPE cyclase system, MPE-cyclase, MPEC, MPEC gene product, MPME cyclase, NTZIP protein, O2-dependent Mg-protoporphyrin monomethyl ester cyclase, OsCRD1, OSZIP protein, oxygen-dependent Mg-protoporphyrin IX monomethylester cyclase system, oxygen-independent Mg-protoporphyrin IX monomethylester cyclase system, PeMPEC, PNZIP protein, RDZIP protein, rice copper response defect 1, SBZIP protein, Sll1214, SOZIP protein, TAZIP protein, TRZIP protein, XanL, Xantha-l, YSL1

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.81 magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase

Cloned

Cloned on EC 1.14.13.81 - magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amin acid sequence determination and analysis, sequence comparisons
gene acsF, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain C43(DE3)
gene CHL27, encoding the CHLH subunit of enzyme Mg protoporphyrin monomethylester cyclase
gene OsCRD1, encoding a putative subunit of enzyme MPEC, is a single-copy gene in rice, sequence comparisons, quantitative real-time RT-PCR enzyme expression analysis
gene PeMPEC, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, recombinant overexpression in Arabidopsis thaliana Col-0 using the Agrobacterium tumefaciens strain GV310 transformation method. Gene PeMPEC driven by the CAMV 35S promoter is transferred into the Crd1 mutant leading to functional complementation of the mutant, the chlorophyll concentration of sense plants is 22-50% higher than that of the Crd1 mutant, and 7-20% higher than that of wild-type Col-0
gene xantha-1, DNA and amino acid sequence determination and analysis
-
gene xantha-1, DNA and amino acid sequence determination and analysis, expression of wild-type and mutants in transgenic barley plants
gene Xantha-I, recombinant expression of active XanL strictly requires co-expression with an additional protein, Ycf54, recombinant expression of His-tagegd XanL and Ycf54 in Escherichia coli strain Artic Express (DE3)
gene ygl8 encodes a catalytic subunit of MgPME cyclase, DNA and amino acid sequence determination and analysis, map-based cloning, sequence comparisons and phylogenetic analysis. Transient expression in Nicotiana benthamiana. The recombinant construct pC1305-YGL8 is introduced into the ygl8 rice mutant by Agrobacterium tumefaciens EHA105-mediated transformation. Quantitative real-time PCR expression analysis
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gene ysl1, DNA and amino acid sequence determination and analysis, genotyping, sequence comparisons and phylogenetic tree, complementation of the ysl1 mutant by complementation construct pC2300-YSL1 using the Agrobacterium tumefaciens transfection method, semiquantitative and quantitative real-time RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in rice protoplasts
genes chlAI, chlAII, slr0905, sll1242, or slr0309, expression of wild-type and mutants enzymes, expression analysis, overview
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PCR-amplification of cDNA from isolated RNA, PCR-products of enzyme-GFP fusion constructs are cloned into vector pPENSOTG and introduced into Arabidopsis protoplasts by polyethylene glycol-mediated transformation, complementation constructs are produced by joining PCR fragments of the enzyme promoter with the enzyme-GFP cDNA, cloned into vector pBIB-HYG, introduced into Agrobacterium GV3101, transformed into homozygous mutant plants via the floral dipping method
RNA is isolated to synthesize cDNA and quantitative real-time PCR is carried out for anaerobic and semiaerobic growth conditions
YL-1 subunit of MPEC by map-based cloning, quantitative real-time PCR expression analysis, phylogenetic analysis
-