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1.14.13.8: flavin-containing monooxygenase

This is an abbreviated version!
For detailed information about flavin-containing monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.8

Reaction

Hypotaurine
+
H2O
 +
NAD+
=
taurine
 +
NADH
 +
H+

Synonyms

class 3 flavin-containing mono-oxygenase, class 3 FMO, dechlorinating flavin-dependent monooxygenase, dimethylaniline monooxygenase (N-oxide-forming), dimethylaniline monooxygenase [N-oxide-forming] 1, dimethylaniline N-oxidase, dimethylaniline oxidase, dimethylsulfone monooxygenase, DMA oxidase, EC 1.13.12.11, EC 1.8.1.3, EtaA, FAD-containing monooxygenase, FAD-containing monooxygenase 3, flavin containing monooxygenase 3, flavin mono-oxygenase, flavin monooxygenase, flavin-containing mono-oxygenase, flavin-containing monooxygenase, flavin-containing monooxygenase 1, flavin-containing monooxygenase 3, flavin-containing monooxygenase 5, flavin-containing monooxygenase-3, flavin-containing-monooxygenase, flavin-dependent monooxygenase, flavoprotein monooxygenase, FMO, FMO 1A1, FMO 1B1, FMO 1C1, FMO 1D1, FMO 1E1, FMO-E, FMO-I, FMO-II, FMO1, FMO2, FMO2.1, FMO3, FMO4, FMO5, FMOGS-OX6, FMOGS-OX7, HadA, hFMO1, hFMO3, hFMO5, Met S-oxidase, mFMO, mixed-function amine oxidase, monooxygenase FMO1, More, MymA, N,N-dimethylaniline monooxygenase, oxygenase, dimethylaniline mono- (N-oxide-forming), oxygenase, methylphenyltetrahydropyridine N-mono-, PtFMO, sfnG, TetX, type II flavin-containing monooxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.8 flavin-containing monooxygenase

Purification

Purification on EC 1.14.13.8 - flavin-containing monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 forms: FMO-I and FMO-II
-
amino acid composition
-
ammonium sulfate precipitation, dialysis
-
amylose column chromatography
-
from liver microsomes
-
isozyme from lung microsomes
-
N-terminal amino acid sequence
-
native enzyme from liver microsomes
-
native enzyme from lung microsomes
-
native FMO3 130fold from kidney microsomes
Ni-NTA column chromatography
nickel affinity column chromatography
-
one-step purification of the recombinant PTDH-mFMO from Escherichia coli by nickel affinity chromatography
-
partial
preparation of liver microsomes
recombinant His-tagged enzyme in Escherichia coli strain BL21(DE3)
recombinant His-tagged EtaA from Escherichia coli strain DH5alpha by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain JM109 by negative anionic exchange chromatography (the protein is not retained by the DEAE resin at pH 7.4) and nickel affinity chromatography, followed by ultrafiltration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain JM109 by nickel affinity chromatography and ultrafiltration
recombinant His-tagged wild-type and mutant full-length and truncated enzyme variants from Escherichia coli strain JM109 by anion exchange and nickel affinity chromatography followed by ultrafiltration
recombinant maltose-binding protein fusion enzymes FMO1, FMO3, and FMO5 from Escherichia coli by amylose affinity chromatography
-
recombinant MBP-fusion FMO3 and FMO5 from Escherichia coli strain DH5alpha to about 90% purity by amylose affinity chromatography, and for FMO3 also further by anion exchange chromatography
-
recombinant protein
recombinant wild-type and mutant FMO3 from Escherichia coli strain JM109 in a multistep process
-
Strep-tag affinity column chromatography
-