Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.13.7: phenol 2-monooxygenase (NADPH)

This is an abbreviated version!
For detailed information about phenol 2-monooxygenase (NADPH), go to the full flat file.

Word Map on EC 1.14.13.7

Reaction

phenol
+
NADPH
+
H+
+
O2
=
catechol
+
NADP+
+
H2O

Synonyms

DmpLNO, flavin containing monooxygenase, LmPH, Mph, MphN, multi-component phenol hydroxylase, multicomponent PH, multicomponent phenol hydroxylase, multicomponent phenol hydroxylase alpha subunit, NCgl2588, oxygenase, phenol 2-mono-, PHE, phenol hydroxylase, phenol o-hydroxylase, PHH, phhY, PHIND, PHO, PHR, single-component PH, SPH

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.7 phenol 2-monooxygenase (NADPH)

Cofactor

Cofactor on EC 1.14.13.7 - phenol 2-monooxygenase (NADPH)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
-
essential component of the enzyme system
-
[2Fe-2S]-center
-
under ideal conditions, the hydroxylated product yield is about 50% of the diiron centers, suggesting that the enzyme operates by half-sites reactivity mechanisms
additional information
-
phenol hydroxylase shows specific ability to use NADH or NADPH or both as cofactors, phenol is hydroxylated to catechol with concomitant oxidation of NADH. The specific activity of phenol hydroxylase reaction with NADPH as cofactor is about 3times higher when compared to specific activity with NADH as cofactor, suggesting NADPH is a better cofactor
-