1.14.13.7: phenol 2-monooxygenase (NADPH)
This is an abbreviated version!
For detailed information about phenol 2-monooxygenase (NADPH), go to the full flat file.
Word Map on EC 1.14.13.7
-
1.14.13.7
-
catechols
-
phenol-degrading
-
hydroxylases
-
2,3-dioxygenase
-
trichosporon
-
cutaneum
-
meta-cleavage
-
diiron
-
cresol
-
comamonas
-
2-hydroxymuconic
-
testosteroni
-
haldane
-
coke
-
radioresistens
-
carboxylate-bridged
-
meta-pathway
-
ortho-cleavage
-
methylococcus
-
cis,cis-muconate
-
coking
-
environmental protection
-
industry
-
synthesis
-
degradation
- 1.14.13.7
- catechols
-
phenol-degrading
- hydroxylases
-
2,3-dioxygenase
- trichosporon
- cutaneum
-
meta-cleavage
-
diiron
- cresol
- comamonas
-
2-hydroxymuconic
- testosteroni
-
haldane
-
coke
- radioresistens
-
carboxylate-bridged
-
meta-pathway
-
ortho-cleavage
-
methylococcus
- cis,cis-muconate
-
coking
- environmental protection
- industry
- synthesis
- degradation
Reaction
Synonyms
DmpLNO, flavin containing monooxygenase, LmPH, Mph, MphN, multi-component phenol hydroxylase, multicomponent PH, multicomponent phenol hydroxylase, multicomponent phenol hydroxylase alpha subunit, NCgl2588, oxygenase, phenol 2-mono-, PHE, phenol hydroxylase, phenol o-hydroxylase, PHH, phhY, PHIND, PHO, PHR, single-component PH, SPH
ECTree
Advanced search results
Cofactor
Cofactor on EC 1.14.13.7 - phenol 2-monooxygenase (NADPH)
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
[2Fe-2S]-center
-
under ideal conditions, the hydroxylated product yield is about 50% of the diiron centers, suggesting that the enzyme operates by half-sites reactivity mechanisms
additional information
-
phenol hydroxylase shows specific ability to use NADH or NADPH or both as cofactors, phenol is hydroxylated to catechol with concomitant oxidation of NADH. The specific activity of phenol hydroxylase reaction with NADPH as cofactor is about 3times higher when compared to specific activity with NADH as cofactor, suggesting NADPH is a better cofactor
-
FAD
-
2 FAD per dimer and 3 FAD per tetramer after removing FAD and reconstituting the apoenzyme with the cofactor
NADH
-
the phenol hydrolxylase component P reduces several artificial electron acceptors such as horse heart cytochrome c, 2,6-dichlorophenolindophenol, and potassium ferricyanide, with either NADH or NADPH as electron donor. NADH is preferentially used
NADPH
-
-
NADPH
-
the phenol hydrolxylase component P reduces several artificial electron acceptors such as horse heart cytochrome c, 2,6-dichlorophenolindophenol, and potassium ferricyanide, with either NADH or NADPH as electron donor. NADH is preferentially used