1.14.13.31: 2-nitrophenol 2-monooxygenase

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For detailed information about 2-nitrophenol 2-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.31

1.14.13.31

catechols

putida

alcaligenes

o-benzoquinone

1,2-dioxygenase

ortho-cleavage

magnesium

manganese

Reaction

+ 2 NADPH + 2 H+ +

+ 2 NADP⁺ +

Synonyms

nitrophenol oxygenase, ONP 2-monooxygenase, OnpA, oxygenase, 2-nitrophenol

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.13.31 2-nitrophenol 2-monooxygenase

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Results	in table
1	AA Sequence
1	CAS Registry Number
1	Cloned(Commentary)
8	Cofactor
2	General Stability
4	Inhibitors
1	Ki Value [mM]
3	KM Value [mM]
2	Localization
7	Metals/Ions
2	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
17	Organism
1	Pathway
3	pH Optimum
1	pH Range
1	pH Stability
2	Purification (Commentary)
1	Reaction
3	Reaction Type
10	Reference
2	Source Tissue
5	Specific Activity [micromol/min/mg]
2	Storage Stability
43	Substrates and Products (Substrate)
2	Subunits
6	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
2	Temperature Stability [°C]

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Cloned on EC 1.14.13.31 - 2-nitrophenol 2-monooxygenase

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Go to Cloned (commentary) Search

genes onpA and onpB, DNA and amino acid sequence determination and analysis, phylogenetic analysis, subcloning and expression in Escherichia coli, co-expression with onpB, but not of onpA alone, in the catechol utilizer Pseudomonas putida strain PaW340 confers the ability to grow on 2-nitrophenol

Alcaligenes sp.

A7KS54

687390