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1.14.13.29: 4-nitrophenol 2-monooxygenase

This is an abbreviated version!
For detailed information about 4-nitrophenol 2-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.29

Reaction

4-nitrophenol
+
NADH
 +
H+
 +
O2
=
4-nitrocatechol
 +
NAD+
 +
H2O

Synonyms

4-nitrophenol 4-monooxygenase/4-nitrocatechol 2-monooxygenase, 4-nitrophenol hydroxylase, 4-nitrophenol-2-hydroxylase, 4-NPH, CYP 2E1, CYP2A/2E1, CYP2E1, cytochrome P-450 2E1, cytochrome P-450 isozyme 3a, NphA1, oxygenase, 4-nitrophenol 2-mono-, p-nitrophenol 2-hydroxylase, p-nitrophenol hydroxylase, p-nitrophenol hydroxylase component A, p-nitrophenol-hydroxylase, PNP 2-monooxygenase, PNP monooxygenase, PnpA1, PNPH, PPH, two-component 4-nitrophenol hydroxylase, two-component PNP monooxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.29 4-nitrophenol 2-monooxygenase

Specific Activity

Specific Activity on EC 1.14.13.29 - 4-nitrophenol 2-monooxygenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000037
-
microsomal fraction
0.000494
-
-
0.00275
-
malaria infected mouse, real time kinetic method
0.00411
-
mouse without infection, real time kinetic method
0.0128
-
substrate 2-chloro-4-nitrophenol, pH 7.6, 22°C
0.0179
-
substrate 4-nitrocatechol, pH 7.6, 22°C
0.034
-
substrate 4-nitrophenol, pH 7.6, 22°C
11.2
cell extract, 0.3 mM 4-nitrophenol as substrate, 1 mM NADH, 5 M FAD, 1 mM DTT, NphA1 (approximately 0.5 mg of purified protein), and NphA2 (approximately 0.1 mg of purified protein) in 1 ml of 50 mM Tris-HCl buffer (pH 7.5), 22°C
12.8
-
substrate 2-chloro-4-nitrophenol, pH 7.6, 23°C
132.8
-
surgically castrated pig, pH 6.8
161.5
-
immunocastrated pig, pH 6.8
17.9
-
substrate 4-nitrocatechol, pH 7.6, 23°C
24.5
HiTrap Q-Sepharose purified enzyme, 0.3 mM 4-nitrophenol as substrate, 1 mM NADH, 5 M FAD, 1 mM DTT, NphA1 (approximately 0.5 mg of purified protein), and NphA2 (approximately 0.1 mg of purified protein) in 1 ml of 50 mM Tris-HCl buffer (pH 7.5), 22°C
34.2
-
substrate 4-nitrophenol, pH 7.6, 23°C
98.3
-
entire male pig, pH 6.8