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1.14.13.25: methane monooxygenase (soluble)

This is an abbreviated version!
For detailed information about methane monooxygenase (soluble), go to the full flat file.

Word Map on EC 1.14.13.25

Reaction

methane
+
NAD(P)H
 +
H+
 +
O2
=
methanol
 +
NAD(P)+
 +
H2O

Synonyms

chcA, cytoplasmic methane monooxygenase, methane hydroxylase, methane mono-oxygenase, methane monooxygenase, methane monooxygenase hydroxylase, MmMmoC, MMO, MMO Bath, MMOB, MmoC, MMOH, MMOR, oxygenase, methane mono-, particulate methane monooxygenase, pMMO, sMMO, soluble methane monooxygenase, soluble methane monooxygenase hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.25 methane monooxygenase (soluble)

Specific Activity

Specific Activity on EC 1.14.13.25 - methane monooxygenase (soluble)

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00011
-
purified enzyme, using propylene and duroquinol as substrates
0.0008
-
sMMO, substrate chloronaphthalene
0.0012
-
sMMO, substrate naphthalene
0.0029
-
membrane-bound enzyme, using propylene and NADH as substrates
0.003
-
membrane-bound enzyme, using propylene and duroquinol as substrates
0.0039
-
sMMO, substrate chloropentane
0.0122
-
sMMO, low copper growth concentration, growth substrate nitrate
0.0191
-
sMMO, substrate butylene
0.02 - 0.04
-
purified MMOH, substrate propylene
0.0254
-
sMMO, substrate propylene
0.0336
-
sMMO, substrate ethylene
0.072
-
component A
0.1
-
about, all 3 components individually
0.185
-
component A
0.205
-
sMMO, substrate diethylic ether
0.208
-
purified component A hydroxylase
0.265
-
protein A after reconstitution of iron
0.3 - 0.45
-
pH 7.0, 45°C
0.334
-
sMMO, substrate propane
0.604
-
purified enzyme, substrate propylene
0.764
-
all components, substrate propene
1.66
-
sMMO protein B triple mutant G10A/G13Q/G16A
11.37
-
purified protein B
17.5
-
component C
2.284
-
His-tagged sMMO protein B triple mutant G10A/G13Q/G16A
26.1
-
purified enzyme, substrate propene
3.99
-
sMMO protein B mutant G13Q
494
-
purified recombinant catalytic subunit MMOH, pH 7.5, 30°C
5.09
-
sMMO, wild-type enzyme
5.37
-
pH 7.4, temperature not specified in the publication
5.71
-
purified component C
additional information