1.14.13.23: 3-hydroxybenzoate 4-monooxygenase

This is an abbreviated version!
For detailed information about 3-hydroxybenzoate 4-monooxygenase, go to the full flat file.

Reaction

Synonyms

3-hydroxybenzoate 4-hydroxylase, 3-hydroxybenzoate hydroxylase, 3-hydroxybenzoate-4-hydroxylase, 3HB4H, EC 1.14.99.13, FAD-dependent 4-hydroxybenzoate hydroxylase, m-hydroxybenzoate hydroxylase, MHBH, MobA, oxygenase, 3-hydroxybenzoate 4-mono-

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.23 3-hydroxybenzoate 4-monooxygenase

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Results	in table
2	Activating Compound
34	AA Sequence
1	CAS Registry Number
4	Cloned(Commentary)
16	Cofactor
2	Crystallization (Commentary)
4	General Information
1	General Stability
14	Inhibitors
15	KM Value [mM]
1	Metals/Ions
3	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
17	Organism
4	Pathway
2	PDB ID
3	pH Optimum
7	Protein Variants
3	Purification (Commentary)
3	Reaction
3	Reaction Type
15	Reference
3	Source Tissue
6	Specific Activity [micromol/min/mg]
32	Substrates and Products (Substrate)
6	Subunits
24	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]

Engineering

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Engineering on EC 1.14.13.23 - 3-hydroxybenzoate 4-monooxygenase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

A400G

Comamonas testosteroni

Q6SSJ6

random mutagenesis, the substitution confers the ability to the mutant enzyme to transform 3-aminophenol to a related substituted catechol

684919

D416A

Comamonas testosteroni

Q6SSJ6

random mutagenesis, the substitution confers the ability to the mutant enzyme to transform 3-aminophenol to a related substituted catechol

684919

H135P

Comamonas testosteroni

Q6SSJ6

random mutagenesis, the substitution confers the ability to the mutant enzyme to transform 3-aminophenol to a related substituted catechol

684919

K326I

Comamonas testosteroni

Q6SSJ6

random mutagenesis, the substitution confers the ability to the mutant enzyme to transform phenol to catechol

684919

V257A

2 entries

additional information

Comamonas testosteroni

Q6SSJ6

screening of random mutants from a cosmid library for altered substrate specificities

684919

V257A

Comamonas testosteroni

Q6SSJ6

random mutagenesis, the substitution confers the ability to the mutant enzyme to transform phenol to catechol, the mutant is also active with resorcinol, hydroquinone, p-hydroxybenzoate, 2,5-dihydroxybenzoate, 3,4-dihydroxybenzoate, 3-chlorophenol, 4-chlorophenol, 4-chlororesorcinol, and 4-nitrophenol

684919

V257A

Comamonas testosteroni

Q6SSJ6

a directed evolution study reveals that 3HB4H from Comamonas testosteroni strain GZ39 is considerably active with 4-hydroxybenzoate and that this activity increases in the V257A variant. The same variant slowly converts phenol to catechol, an activity not observed with the wild-type enzyme

764036