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1.14.13.23: 3-hydroxybenzoate 4-monooxygenase

This is an abbreviated version!
For detailed information about 3-hydroxybenzoate 4-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.23

Reaction

3-hydroxybenzoate
+
NADPH
+
H+
+
O2
=
3,4-dihydroxybenzoate
+
NADP+
+
H2O

Synonyms

3-hydroxybenzoate 4-hydroxylase, 3-hydroxybenzoate hydroxylase, 3-hydroxybenzoate-4-hydroxylase, 3HB4H, EC 1.14.99.13, FAD-dependent 4-hydroxybenzoate hydroxylase, m-hydroxybenzoate hydroxylase, MHBH, MobA, oxygenase, 3-hydroxybenzoate 4-mono-

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.23 3-hydroxybenzoate 4-monooxygenase

Crystallization

Crystallization on EC 1.14.13.23 - 3-hydroxybenzoate 4-monooxygenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1.4-1.6 M ammonium sulfate, 4-8% dioxane, pH 6.5
-
purified native enzyme in complex with substrate 3-hydroxybenzoate or inhibitor 4-chloromercuribenzoate, and as Xe-derivative, sitting drop vapour diffusion method, 10 mg/ml in 25 mM phosphate buffer, pH 7.5, containing 0.3 mM 3-hydroxybenzoate, mixed with an equal volume of a reservoir solution consisting of 0.1 M MES, pH 6.5, 1.3 M ammonium sulfate, and 6% v/v 1,4-dioxane, 20°C, X-ray diffraction structure determination and analysis at 1.8 A and 2.5 A resolution, respectively