1.14.13.23: 3-hydroxybenzoate 4-monooxygenase
This is an abbreviated version!
For detailed information about 3-hydroxybenzoate 4-monooxygenase, go to the full flat file.
Word Map on EC 1.14.13.23
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1.14.13.23
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phenol
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4-hydroxybenzoate
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hydroxylases
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testosteroni
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fad
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gentisate
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comamonas
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mobility-shift
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salicylate
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repressor
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regioselective
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flavoprotein
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unbound
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mobr
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dioxygenase
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protocatechuate
- 1.14.13.23
- phenol
- 4-hydroxybenzoate
- hydroxylases
- testosteroni
- fad
- gentisate
-
comamonas
-
mobility-shift
- salicylate
- repressor
-
regioselective
- flavoprotein
-
unbound
- mobr
- dioxygenase
- protocatechuate
Reaction
Synonyms
3-hydroxybenzoate 4-hydroxylase, 3-hydroxybenzoate hydroxylase, 3-hydroxybenzoate-4-hydroxylase, 3HB4H, EC 1.14.99.13, FAD-dependent 4-hydroxybenzoate hydroxylase, m-hydroxybenzoate hydroxylase, MHBH, MobA, oxygenase, 3-hydroxybenzoate 4-mono-
ECTree
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Crystallization
Crystallization on EC 1.14.13.23 - 3-hydroxybenzoate 4-monooxygenase
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purified native enzyme in complex with substrate 3-hydroxybenzoate or inhibitor 4-chloromercuribenzoate, and as Xe-derivative, sitting drop vapour diffusion method, 10 mg/ml in 25 mM phosphate buffer, pH 7.5, containing 0.3 mM 3-hydroxybenzoate, mixed with an equal volume of a reservoir solution consisting of 0.1 M MES, pH 6.5, 1.3 M ammonium sulfate, and 6% v/v 1,4-dioxane, 20°C, X-ray diffraction structure determination and analysis at 1.8 A and 2.5 A resolution, respectively