1.14.13.195: L-ornithine N5-monooxygenase (NADPH)
This is an abbreviated version!
For detailed information about L-ornithine N5-monooxygenase (NADPH), go to the full flat file.
Word Map on EC 1.14.13.195
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1.14.13.195
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flavin
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fumigatus
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aspergillus
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flavin-dependent
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pyoverdine
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nadp+
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c4a-hydroperoxyflavin
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hydroxamate
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hydroxamate-containing
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flavin-containing
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non-ribosomal
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half-reaction
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n-hydroxylating
- 1.14.13.195
- flavin
- fumigatus
- aspergillus
-
flavin-dependent
-
pyoverdine
- nadp+
-
c4a-hydroperoxyflavin
- hydroxamate
-
hydroxamate-containing
-
flavin-containing
-
non-ribosomal
-
half-reaction
-
n-hydroxylating
Reaction
Synonyms
Af-OMO, CchB, flavin-dependent monooxygenase, L-Orn N5-oxygenase, L-ornithine N5-hydroxylase, L-ornithine-Ndelta-oxygenase, monooxygenase EtcB, omega-amino acid monooxygenase, ornithine hydroxylase, ornithine N5-monooxygenase, PsbA, PvdA, SidA, siderophore A
ECTree
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Purification
Purification on EC 1.14.13.195 - L-ornithine N5-monooxygenase (NADPH)
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recombinant His-tagged enzyme from Escherichia coli strain Rosetta2(DE3) by nickel affinity chromatography, the tag is cleaved off by thrombin
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recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)-T1 by affinity chromatography, proteolytic tag removal
recombinant N-terminally His-tagged full-length and truncated wild-type and mutant enzymes from Escherichia coli strain M15 by nickel affinity chromatography
recombinant N-terminally thioredoxin-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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