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1.14.13.180: aklavinone 12-hydroxylase

This is an abbreviated version!
For detailed information about aklavinone 12-hydroxylase, go to the full flat file.

Reaction

aklavinone
+
NADPH
+
H+
+
O2
=
epsilon-rhodomycinone
+
NADP+
+
H2O

Synonyms

aklavinone 12-hydroxylase, DnrF, RdmE

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.180 aklavinone 12-hydroxylase

Engineering

Engineering on EC 1.14.13.180 - aklavinone 12-hydroxylase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
M56A
2fold increase in synthesis of kermesic acid
P217K
2.2fold increase in synthesis of kermesic acid
P217K/M56A
significant decrease in synthesis of kermesic acid
R373X
mutants at position Arg373 retain catalytic activity close to wild-type level
Y224F
inactive mutant enzyme