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1.14.13.1: salicylate 1-monooxygenase

This is an abbreviated version!
For detailed information about salicylate 1-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.1

Reaction

salicylate
+
NADH
+
H+
+
O2
=
catechol
+
NAD+
+
H2O
+
CO2

Synonyms

Efe-shyA, FAD/NADH-dependent SA 1-hydroxylase, FAD_binding_3 domain-containing protein, FGSG_03657, FgShy1, FgShyC, flavin-dependent monooxygenase salicylate hydroxylase, NahG, NahU, NRRL3_9723, oxygenase, salicylate 1-mono-, SA 1-decarboxylase/hydroxylase, SA 1-hydroxylase, SA hydroxylase, SA1H, SahA, salA, salcylic acid 1-hydroxylase, SALH, salicylate 1-hydroxylase, Salicylate 1-monooxygenase, salicylate hydroxylase, salicylate hydroxylase (decarboxylating), salicylate monooxygenase, salicylate, NADH: oxygen oxidoreductase, salicylate, NADH: oxygen oxidoreductase (1-hydroxylating, decarboxylating), salicylate-1-hydroxylase, salicylic acid hydroxylase, salicylic hydroxylase, SalOH, SHL, Shy1, ShyA, ShyC, SlSA 1H, SlSA1H, Solyc08g063130

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.1 salicylate 1-monooxygenase

Purification

Purification on EC 1.14.13.1 - salicylate 1-monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography
-
cells grown on 1-hydroxy-2-naphthoate
-
DEAE-Toyopearl 650M column chromatography, phenyl Sepharose CL6B column chromatography, and Sephacryl gel filtration
HisTrap column chromatography
-
native enzyme from 5-chlorosalicylate-grown cells by anion exchange chromatography and gel filtration
Ni-NTA column chromatography and Superdex 75 gel filtration
-
Ni-NTA His Bind resin column chromatography
-
oxygenase component PhnII
-
recombinant expression of His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography, tag cleavage and a second step of nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
TALON metal affinity resin column chromatography