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1.14.12.24: 2,4-dinitrotoluene dioxygenase

This is an abbreviated version!
For detailed information about 2,4-dinitrotoluene dioxygenase, go to the full flat file.

Word Map on EC 1.14.12.24

Reaction

2,4-dinitrotoluene
+
NADH
+
O2
=
4-methyl-5-nitrocatechol
+
nitrite
+
NAD+

Synonyms

2,4-dinitrotoluene dioxygenase, 2,4-DNT dioxygenase, 2,4-DNTDO, DDO, DNT dioxygenase, dntA, DntAc, DNTDO

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
                1.14.12.24 2,4-dinitrotoluene dioxygenase

Specific Activity

Specific Activity on EC 1.14.12.24 - 2,4-dinitrotoluene dioxygenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.38
Q5BM20; Q6QUY4; Q45695; Q6QUY3
with naphthalene as substrate, pH and temperature not specified in the publication
19
-
crude enzyme, at pH 7.2 and 37°C
2.4
-
crude extract, at pH 7.0 and 37°C
37.3
-
after 15.7fold, at pH 7.0 and 37°C
385
-
after 20.3fold purification, at pH 7.2 and 37°C