1.14.12.15: terephthalate 1,2-dioxygenase

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For detailed information about terephthalate 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.14.12.15

1.14.12.15

comamonas

testosteroni

protocatechuate

rieske

ring-hydroxylating

Reaction

(1R,6S)-dihydroxycyclohexa-2,4-diene-1,4-dicarboxylate

Synonyms

1,4-Dicarboxybenzoate 1,2-dioxygenase, Benzene-1,4-dicarboxylate 1,2-dioxygenase, Oxygenase, terephthalate, 1,2-di, TER dioxygenase system, TERDOS, TPA 1,2-dioxygenase, TPADO

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

1.14.12.15 terephthalate 1,2-dioxygenase

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Results	in table
117	AA Sequence
1	CAS Registry Number
2	Cloned(Commentary)
9	Cofactor
2	General Information
3	Inhibitors
3	KM Value [mM]
5	Metals/Ions
15	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
22	Organism
4	Pathway
18	PDB ID
2	pH Optimum
1	pH Stability
2	pI Value
3	Purification (Commentary)
1	Reaction
2	Reaction Type
12	Reference
2	Source Tissue
3	Specific Activity [micromol/min/mg]
24	Substrates and Products (Substrate)
8	Subunits
16	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Stability [°C]

Cloned

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Cloned on EC 1.14.12.15 - terephthalate 1,2-dioxygenase

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expressed in Escherichia coli BL21 (DE3) cells

Comamonas sp.

671450

His-tag fused tphA1II and His-tag fused tphA2IIA3II genes expressed in Escherichia coli BL21(DE3) harboring pET-16tpa1 and pET-16tpa23 respectively, under the control of the T7 promoter

Comamonas sp.

702731