1.14.12.10: benzoate 1,2-dioxygenase
This is an abbreviated version!
For detailed information about benzoate 1,2-dioxygenase, go to the full flat file.
Word Map on EC 1.14.12.10
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1.14.12.10
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catechol
-
calcoaceticus
-
toluate
-
2-chlorobenzoate
-
chlorocatechols
-
rieske
-
cis-diols
-
rieske-type
-
beta-ketoadipate
-
benzoate-grown
-
radioresistens
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3-methylbenzoate
-
benzoate-induced
-
3,4-dioxygenase
-
cis-dihydrodiols
-
4-chlorocatechol
-
ring-hydroxylating
-
arvilla
-
synthesis
- 1.14.12.10
- catechol
- calcoaceticus
- toluate
- 2-chlorobenzoate
- chlorocatechols
-
rieske
-
cis-diols
-
rieske-type
-
beta-ketoadipate
-
benzoate-grown
- radioresistens
- 3-methylbenzoate
-
benzoate-induced
-
3,4-dioxygenase
-
cis-dihydrodiols
- 4-chlorocatechol
-
ring-hydroxylating
- arvilla
- synthesis
Reaction
Synonyms
BDO, BenA, benABC, benzoate 1,2-dioxygenase, benzoate 1,2-dioxygenase oxygenase, benzoate 1,2-dioxygenase system, benzoate dioxygenase, benzoate hydroxylase, benzoic hydroxylase, BZDO, BZDO enzyme, EC 1.13.99.2, oxygenase, benzoate 1,2-di-
ECTree
1.14.12.10 benzoate 1,2-dioxygenaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 1.14.12.10 - benzoate 1,2-dioxygenase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
benzene + O2 + NADH
(R,S)-1,2-dihydro-1,2-dihydroxycatechol + NAD+
-
-
-
-
?
benzoate + NADH-cytochrome c reductase component + H+ + O2
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+-cytochrome c reductase component
-
-
-
-
r
2-methylbenzoate + O2 + NADH
cis-1,2-dihydro-1,2-dihydroxy-2-methyl-benzoate + NAD+
-
-
-
-
?
2-methylbenzoate + O2 + NADH
cis-1,2-dihydro-1,2-dihydroxy-2-methyl-benzoate + NAD+
-
-
-
-
?
3-aminobenzoate + O2 + NADH
3-amino-2-hydro-1,2-dihydroxybenzoate
-
-
-
?
3-aminobenzoate + O2 + NADH
3-amino-2-hydro-1,2-dihydroxybenzoate
-
-
-
?
3-aminobenzoate + O2 + NADH
3-amino-2-hydro-1,2-dihydroxybenzoate
-
activity in E. coli expressing a presumed dioxygenase component from Rhodococcus
-
?
3-aminobenzoate + O2 + NADH
3-amino-2-hydro-1,2-dihydroxybenzoate
-
activity in E. coli expressing a presumed dioxygenase component from Rhodococcus
-
?
3-chlorobenzoate + O2 + NADH + H+
3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
3-chlorobenzoate + O2 + NADH + H+
3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
3-chlorobenzoate + O2 + NADH + H+
3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
?
3-chlorobenzoate + O2 + NADH + H+
3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
3-chlorobenzoate + O2 + NADH + H+
3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
?
3-chlorobenzoate + O2 + NADH + H+
3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-
-
?
3-fluorobenzoate + O2 + NADH + H+
3-fluoro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
3-fluorobenzoate + O2 + NADH + H+
3-fluoro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
benzoate analogues with substituents in meta position are oxygenated more rapidly than those with ortho- or para substitution
-
?
3-fluorobenzoate + O2 + NADH + H+
3-fluoro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
benzoate analogues with substituents in meta position are oxygenated more rapidly than those with ortho- or para substitution
-
?
3-fluorobenzoate + O2 + NADH + H+
3-fluoro-2-hydro-1,2-dihydroxybenzoate + NAD+
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-
-
?
3-methylbenzoate + O2 + NADH + H+
3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
3-methylbenzoate + O2 + NADH + H+
3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
3-methylbenzoate + O2 + NADH + H+
3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
?
3-methylbenzoate + O2 + NADH + H+
3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
3-methylbenzoate + O2 + NADH + H+
3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-
-
?
3-methylbenzoate + O2 + NADH + H+
3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
-
activity in E. coli expressing a presumed dioxygenase component from Rhodococcus
-
?
3-methylbenzoate + O2 + NADH + H+
3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
-
activity in E. coli expressing a presumed dioxygenase component from Rhodococcus
-
?
4-fluorobenzoate + O2 + NADH
4-fluoro-2-hydro-1,2-dihydroxybenzoate
-
-
-
?
4-fluorobenzoate + O2 + NADH
4-fluoro-2-hydro-1,2-dihydroxybenzoate
-
-
-
?
benzoate + NADH + H+ + O2
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
-
-
?
benzoate + NADH + H+ + O2
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
-
-
?
benzoate + NADH + H+ + O2
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-
-
?
benzoate + NADH + H+ + O2
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
-
-
?
benzoate + NADH + H+ + O2
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
-
-
?
benzoate + NADH + H+ + O2
1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate + NAD+
-
-
-
-
?
benzoate + NADH + H+ + O2
1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate + NAD+
-
-
-
-
?
benzoate + NADH + H+ + O2
cis-1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylate + NAD+
-
-
Recombinant strain with overexpression of benABC gene and benD knockout. Highest biotransformation is found with cells grown on 2.88 g/l Na-benzoate, yielding approximately 2.3 g/l 1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylic acid with a yield of 73%. The biotransformation yield decreases along with the increase in substrate benzoate concentration. The benD knockout mutant harboring BZDO genes produced concentration of cis-diol product in fermentation (17 g/l, 61% yield).
-
?
benzoate + NADH + H+ + O2
cis-1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylate + NAD+
-
-
Recombinant strain with overexpression of benABC gene and benD knockout. Highest biotransformation is found with cells grown on 2.88 g/l Na-benzoate, yielding approximately 2.3 g/l 1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylic acid with a yield of 73%. The biotransformation yield decreases along with the increase in substrate benzoate concentration. The benD knockout mutant harboring BZDO genes produced concentration of cis-diol product in fermentation (17 g/l, 61% yield).
-
?
benzoate + O2 + NADH
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
benzoate + O2 + NADH
2-hydro-1,2-dihydroxybenzoate + NAD+
-
catabolism of aromatic compounds
-
-
?
benzoate + O2 + NADH
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
benzoate + O2 + NADH
2-hydro-1,2-dihydroxybenzoate + NAD+
-
catabolism of aromatic compounds
-
-
?
benzoate + O2 + NADH
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
benzoate + O2 + NADH
2-hydro-1,2-dihydroxybenzoate + NAD+
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-
-
?
benzoate + O2 + NADH + H+
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
?
benzoate + O2 + NADH + H+
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
?
benzoate + O2 + NADH + H+
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
?
benzoate + O2 + NADH + H+
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
?
benzoate + O2 + NADH + H+
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
benzoate + O2 + NADH + H+
2-hydro-1,2-dihydroxybenzoate + NAD+
-
activity in E. coli expressing a presumed dioxygenase component from Rhodococcus
-
-
?
benzoate + O2 + NADH + H+
2-hydro-1,2-dihydroxybenzoate + NAD+
-
activity in E. coli expressing a presumed dioxygenase component from Rhodococcus
-
-
?
additional information
?
-
-
benzoate induces the expression of benzoate 1,2-dioxygenase
-
-
?
additional information
?
-
-
benzoate 1,2 dioxygenase oxygenase component (BZDO) prepared in a form with the Rieske cluster oxidized and the mononuclear iron in the Fe(III) state can utilize H2O2 as a source of reduced oxygen to form the correct cis-dihydrodiol product from benzoate
-
-
?
additional information
?
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
benzoate 1,2 dioxygenase oxygenase component (BZDO) prepared in a form with the Rieske cluster oxidized and the mononuclear iron in the Fe(III) state can utilize H2O2 as a source of reduced oxygen to form the correct cis-dihydrodiol product from benzoate
-
-
?
