1.14.12.10: benzoate 1,2-dioxygenase

This is an abbreviated version!
For detailed information about benzoate 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.14.12.10

1.14.12.10

catechol

calcoaceticus

toluate

2-chlorobenzoate

chlorocatechols

rieske

cis-diols

rieske-type

beta-ketoadipate

benzoate-grown

radioresistens

3-methylbenzoate

benzoate-induced

3,4-dioxygenase

cis-dihydrodiols

4-chlorocatechol

ring-hydroxylating

arvilla

synthesis

Reaction

(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate

Synonyms

BDO, BenA, benABC, benzoate 1,2-dioxygenase, benzoate 1,2-dioxygenase oxygenase, benzoate 1,2-dioxygenase system, benzoate dioxygenase, benzoate hydroxylase, benzoic hydroxylase, BZDO, BZDO enzyme, EC 1.13.99.2, oxygenase, benzoate 1,2-di-

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

1.14.12.10 benzoate 1,2-dioxygenase

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Results	in table
1824	AA Sequence
3	Application
1	CAS Registry Number
5	Cloned(Commentary)
15	Cofactor
1	General Stability
3	Inhibitors
3	Ki Value [mM]
9	KM Value [mM]
8	Metals/Ions
16	Molecular Weight [Da]
17	Natural Substrates/ Products (Substrates)
151	Organism
7	Pathway
1	PDB ID
4	pH Optimum
1	pH Range
2	pI Value
6	Purification (Commentary)
5	Reaction
6	Reaction Type
30	Reference
5	Specific Activity [micromol/min/mg]
1	Storage Stability
66	Substrates and Products (Substrate)
12	Subunits
28	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
6	Turnover Number [1/s]

Purification

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Purification on EC 1.14.12.10 - benzoate 1,2-dioxygenase

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benzoate oxygenase and reductase component

Pseudomonas putida

396142

DEAE-Sepharose Fast Flow column chromatography and Ultrogel AcA 34 gel filtration

Pseudomonas putida

396142

Hi Trap SP-FF Sepharose column chromatography

oxygenase component

Q-Sepharose column chromatography and Superdex 200 GPC column gel filtration

Pseudomonas putida

677068

recombinant and native enzyme

Acinetobacter calcoaceticus

659031