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1.14.11.B16: carotenoid 4,4'-beta-ionone ring oxygenase

This is an abbreviated version!
For detailed information about carotenoid 4,4'-beta-ionone ring oxygenase, go to the full flat file.

Word Map on EC 1.14.11.B16

Reaction

beta-carotene
+
2-oxoglutarate
 +
O2
 +
H2O
=
canthaxanthin
 +
succinate
 +
CO2
 + 4 H+

Synonyms

4,(4')-beta-ionone ring oxygenase, 4,4' beta-oxygenase, 4,4' carotenoid oxygenase, 4,4'beta-oxygenase, beta-C-4-oxygenase, beta-carotene ketolase, bkt, carotene ketolase, CrtW

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.B16 carotenoid 4,4'-beta-ionone ring oxygenase

KM Value

KM Value on EC 1.14.11.B16 - carotenoid 4,4'-beta-ionone ring oxygenase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0143
3-hydroxyechinenone
30°C, 0.4 M Tris/HCl, pH 8.0, 1 mM dithiothreitol, 0.5 mM FeSO4, 5 mM ascorbic acid, 0.5 mM 2-oxoglutarate, 0.1% (mass/vol) deoxycholate
0.0083
4-oxozeaxanthin
30°C, 0.4 M Tris/HCl, pH 8.0, 1 mM dithiothreitol, 0.5 mM FeSO4, 5 mM ascorbic acid, 0.5 mM 2-oxoglutarate, 0.1% (mass/vol) deoxycholate
0.0013
beta-carotene
30°C, 0.4 M Tris/HCl, pH 8.0, 1 mM dithiothreitol, 0.5 mM FeSO4, 5 mM ascorbic acid, 0.5 mM 2-oxoglutarate, 0.1% (mass/vol) deoxycholate
0.002
echinenone
30°C, 0.4 M Tris/HCl, pH 8.0, 1 mM dithiothreitol, 0.5 mM FeSO4, 5 mM ascorbic acid, 0.5 mM 2-oxoglutarate, 0.1% (mass/vol) deoxycholate
0.01
zeaxanthin
30°C, 0.4 M Tris/HCl, pH 8.0, 1 mM dithiothreitol, 0.5 mM FeSO4, 5 mM ascorbic acid, 0.5 mM 2-oxoglutarate, 0.1% (mass/vol) deoxycholate