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1.14.11.9: flavanone 3-dioxygenase

This is an abbreviated version!
For detailed information about flavanone 3-dioxygenase, go to the full flat file.

Word Map on EC 1.14.11.9

Reaction

a (2S)-flavan-4-one
+
2-oxoglutarate
+
O2
=
a (2R,3R)-dihydroflavonol
+
succinate
+
CO2

Synonyms

(2S)-flavanone 3-hydroxylase, AaF3H, AcF3H, BnF3H, CsF3H, CtF3H, F3H, F3H protein, F3H1, F3H2, FHT, FHTPH, flavanone 3-dioxygenase, flavanone 3-hydroxylase, flavanone 3beta-hydroxylase, flavanone synthase I, flavanone-3-hydroxylase, FLS1, FLS2, FS I, LcF3H, naringenin 3-dioxygenase, naringenin,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating), oxygenase, flavanone 3-di-, PeF3H, PgF3H, PnF3H, RtF3H1, RtF3H2, VcF3H

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.9 flavanone 3-dioxygenase

Engineering

Engineering on EC 1.14.11.9 - flavanone 3-dioxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotecnology
-
AaF3H is a potential target for regulation of flavonoids biosynthesis in Artemisia annua through metabolic engineering
D195E
-
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol
D195E/L215V/K216R
-
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol
D331H
-
no catalytic activity
I115T
-
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol
I115T/V116I
-
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol
I115T/V116I/I131F/D195E
-
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is converted to 76% flavanone 3beta-hydroxylase product dihydrokaempferol and to 24% flavone synthase product apigenin
I131F
-
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol
I131F/D195E
-
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol
I131F/D195E/L215V/K216R
-
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is converted to 69% flavanone 3beta-hydroxylase product dihydrokaempferol and to 31% flavone synthase product apigenin
I131F/L215V/K216R
-
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is converted to 78% flavanone 3beta-hydroxylase product dihydrokaempferol and to 22% flavone synthase product apigenin
L215V/K216R
-
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol
M106T
-
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is completely converted to flavanone 3beta-hydroxylase product dihydrokaempferol
M106T/I115T/V116I/I131F/D195E
-
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is converted to 66% flavanone 3beta-hydroxylase product dihydrokaempferol and to 34% flavone synthase product apigenin
M106T/I115T/V116I/I131F/D195E/L215V/K216R
-
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is converted to 18% flavanone 3beta-hydroxylase product dihydrokaempferol and to 82% flavone synthase product apigenin
M106T/I131F/D195E
-
mutant constructed to confer flavone synthase activity to flavanone 3beta-hydroxylase. Substrate naringenin is converted to 85% flavanone 3beta-hydroxylase product dihydrokaempferol and to 15% flavone synthase product apigenin
H220Q
-
catalytic activity is reduced to about 0.15% of that of the wild-type enzyme. Slightly increased Km-value with respect to iron binding, as compared to the wild-type enzyme
H278Q
-
mutant enzyme has no detectable enzyme activity
N222N
-
catalytic activity is reduced to about 0.15% of that of the wild-type enzyme. Slightly increased Km-value with respect to iron binding, as compared to the wild-type enzyme
R288K
-
decrease in catalytic activity and a 5fold increase in Km-value for 2-oxoglutarate
R288Q
-
decrease in catalytic activity and a 160fold increase in Km-value for 2-oxoglutarate
S290A
-
activity is reduced to 8% of that of the wild-type enzyme
S290T
-
activity is reduced to 20% of that of the wild-type enzyme
S290V
-
activity is reduced to 1% of that of the wild-type enzyme
additional information