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1.14.11.48: xanthine dioxygenase

This is an abbreviated version!
For detailed information about xanthine dioxygenase, go to the full flat file.

Reaction

xanthine
+
2-oxoglutarate
+
O2
=
Urate
+
succinate
+
CO2

Synonyms

alpha-ketoglutarate-dependent xanthine hydroxylase, AO090001000152, TauD domain-containing protein, XanA, xanthine/alpha-ketoglutarate dioxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.48 xanthine dioxygenase

Storage Stability

Storage Stability on EC 1.14.11.48 - xanthine dioxygenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, stable for at least 2 months
-
4°C, stored in 100 mM Tris buffer (pH 7) containing 300 mM NaCl, 250 mM imidazole, 15% glycerol, and 0.005 mM Fe2+, concentrated enzyme is stable for at least 5 months
-
4°C, stored in 100 mM Tris buffer (pH 8.0) containing 300 mM NaCl, 250 mM imidazole, 1 mM EDTA, and 15% glycerol, stable for at least 1 month
-