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1.14.11.44: (R)-dichlorprop dioxygenase (2-oxoglutarate)

This is an abbreviated version!
For detailed information about (R)-dichlorprop dioxygenase (2-oxoglutarate), go to the full flat file.

Word Map on EC 1.14.11.44

Reaction

(R)-(2,4-dichlorophenoxy)propanoate
+
2-oxoglutarate
+
O2
=
2,4-Dichlorophenol
+
pyruvate
+
succinate
+
CO2

Synonyms

(R)-phenoxypropionate/alpha-ketoglutarate-dioxygenase, 2-oxoglutarate-dependent (R)-dichlorprop dioxygenase, AAD-1, alpha-ketoglutarate-dependent (R)-dichlorprop dioxygenase, ariloxyalkanoate-dioxygenase-1, RdpA

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.44 (R)-dichlorprop dioxygenase (2-oxoglutarate)

Engineering

Engineering on EC 1.14.11.44 - (R)-dichlorprop dioxygenase (2-oxoglutarate)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F171A
exhibits about 70% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 245% compared to the wild-type value
F171Q
exhibits about 70% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 70% compared to the wild-type value
I106A
exhibits about 30% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 5% compared to the wild-type value
I106G/G107I
exhibits 0.7% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate
I106G/G107N
exhibits 8.7% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate
L83A
exhibits about 10% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate
Q93A
exhibits about 60% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 14% compared to the wild-type value
R285A
V80A
exhibits about 60% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 17% compared to the wild-type value
Y221A