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1.14.11.43: (S)-dichlorprop dioxygenase (2-oxoglutarate)

This is an abbreviated version!
For detailed information about (S)-dichlorprop dioxygenase (2-oxoglutarate), go to the full flat file.

Reaction

(S)-(2,4-dichlorophenoxy)propanoate
+
2-oxoglutarate
+
O2
=
2,4-Dichlorophenol
+
pyruvate
+
succinate
+
CO2

Synonyms

(S)-phenoxypropionate/alpha-ketoglutarate-dioxygenase, 2-oxoglutarate-dependent (S)-dichlorprop dioxygenase, alpha-ketoglutarate-dependent (S)-dichlorprop dioxygenase, SdpA

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.43 (S)-dichlorprop dioxygenase (2-oxoglutarate)

Engineering

Engineering on EC 1.14.11.43 - (S)-dichlorprop dioxygenase (2-oxoglutarate)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E69A
exhibits 30.8% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 42% compared to the wild-type value
G97I/N98G
exhibits 3.8% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 0.6% compared to the wild-type value
G97N/N98G
exhibits 0.3% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate
H208A
exhibits about 1.4% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 0.1% compared to the wild-type value
H272A
exhibits about 1.2% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate
Q162F
exhibits about 4.8% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate
R207A
exhibits about 17.3% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 2% compared to the wild-type value
R207V
exhibits about 2.8% of wild-type enzyme activity with (S)-2-(4-chloro-2-methylphenoxy)propanoate
R274A
inactive mutant enzyme