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1.14.11.26: deacetoxycephalosporin-C hydroxylase

This is an abbreviated version!
For detailed information about deacetoxycephalosporin-C hydroxylase, go to the full flat file.

Word Map on EC 1.14.11.26

Reaction

deacetoxycephalosporin C
+
2-oxoglutarate
+
O2
=
deacetylcephalosporin C
+
succinate
+
CO2

Synonyms

cefF, Cephalosporin biosynthesis expandase/hydroxylase, DACS, DAOC hydroxylase, DAOC synthase, DAOC/DAC synthase, DAOCS, deacetoxycephalosporin C hydroxylase, deacetoxycephalosporin C synthase, deacetylcephalosporin C synthase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.26 deacetoxycephalosporin-C hydroxylase

Reference

Reference on EC 1.14.11.26 - deacetoxycephalosporin-C hydroxylase

Please use the Reference Search for a specific query.
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ghag, S.K.; Brems, D.N.; Hassell, T.C.; Yeh, W.K.
Refolding and purification of Cephalosporium acremonium deacetoxycephalosporin C synthetase/hydroxylase from granules of recombinant Escherichia coli
Biotechnol. Appl. Biochem.
24
109-119
1996
Acremonium chrysogenum
Manually annotated by BRENDA team
Dotzlaf, J.E.; Yeh, W.K.
Purification and properties of deacetoxycephalosporin C synthase from recombinant Escherichia coli and its comparison with the native enzyme purified from Streptomyces clavuligerus
J. Biol. Chem.
264
10219-10227
1989
Streptomyces clavuligerus
Manually annotated by BRENDA team
Lloyd, M.D.; Lipscomb, S.J.; Hewitson, K.S.; Hensgens, C.M.H.; Baldwin, J.E.; Schofield, C.J.
Controlling the substrate selectivity of deacetoxycephalosporin/deacetylcephalosporin C synthase
J. Biol. Chem.
279
15420-15426
2004
Acremonium chrysogenum
Manually annotated by BRENDA team
Coque, J.J.; Enguita, F.J.; Cardoza, R.E.; Martin, J.F.; Liras, P.
Characterization of the cefF gene of Nocardia lactamdurans encoding a 3'-methylcephem hydroxylase different from the 7-cephem hydroxylase
Appl. Microbiol. Biotechnol.
44
605-609
1996
Amycolatopsis lactamdurans
Manually annotated by BRENDA team
Samson, S.M.; Dotzlaf, J.E.; Slisz, M.L.; Becker, G.W.; Van Frank, R.M.; Veal, L.E.; Yeh, W.K.; Miller, J.R.; Queener, S.W.; Ingolia, T.D.
Cloning and expression of the fungal expandase/hydroxylase gene involved in cephalosporin biosynthesis
Bio/Technology
5
1207-1214
1987
Acremonium chrysogenum
-
Manually annotated by BRENDA team
Baldwin, J.E.; Adlington, R.M.; Coates, J.B.; Crabbe, M.J.; Crouch, N.P.; Keeping, J.W.; Knight, G.C.; Schofield, C.J.; Ting, H.H.; Vallejo, C.A.; et al.
Purification and initial characterization of an enzyme with deacetoxycephalosporin C synthetase and hydroxylase activities
Biochem. J.
245
831-841
1987
Acremonium chrysogenum
Manually annotated by BRENDA team
Wu, X.B.; Fan, K.Q.; Wang, Q.H.; Yang, K.Q.
C-terminus mutations of Acremonium chrysogenum deacetoxy/deacetylcephalosporin C synthase with improved activity toward penicillin analogs
FEMS Microbiol. Lett.
246
103-110
2005
Acremonium chrysogenum
Manually annotated by BRENDA team
Jensen, S.E.; Westlake, D.W.S.; Wolfe, S.
Deacetoxycephalosporin C synthetase and deacetoxycephalosporin C hydroxylase are two separate enzymes in Streptomyces clavuligerus
J. Antibiot.
38
263-265
1985
Streptomyces clavuligerus
Manually annotated by BRENDA team
Dotzlaf, J.E.; Yeh, W.K.
Copurification and characterization of deacetoxycephalosporin C synthetase/hydroxylase from Cephalosporium acremonium
J. Bacteriol.
169
1611-1618
1987
Acremonium chrysogenum
Manually annotated by BRENDA team
Baker, B.J.; Dotzlaf, J.E.; Yeh, W.K.
Deacetoxycephalosporin C hydroxylase of Streptomyces clavuligerus. Purification, characterization, bifunctionality, and evolutionary implication
J. Biol. Chem.
266
5087-5093
1991
Streptomyces clavuligerus
Manually annotated by BRENDA team
Stok, J.E.; Baldwin, J.E.
Development of enzyme-linked immunosorbent assays for the detection of deacetoxycephalosporin C and isopenicillin N synthase activity
Anal. Chim. Acta
577
153-162
2006
Streptomyces clavuligerus
Manually annotated by BRENDA team
Wei, C.; Yang, Y.; Deng, C.; Liu, W.; Hsu, J.; Lin, Y.; Liaw, S.; Tsai, Y.
Directed evolution of Streptomyces clavuligerus deacetoxycephalosporin C synthase for enhancement of penicillin G expansion
Appl. Environ. Microbiol.
71
8873-8880
2005
Streptomyces clavuligerus
Manually annotated by BRENDA team
Goo, K.S.; Chua, C.S.; Sim, T.S.
Directed evolution and rational approaches to improving Streptomyces clavuligerus deacetoxycephalosporin C synthase for cephalosporin production
J. Ind. Microbiol. Biotechnol.
36
619-633
2009
Streptomyces clavuligerus (P42220)
Manually annotated by BRENDA team
Liras, P.; Demain, A.L.
Enzymology of beta-lactam compounds with cephem structure produced by actinomycete
Methods Enzymol.
458
401-429
2009
Actinomyces sp.
Manually annotated by BRENDA team
An, Y.; Dong, H.; Liu, G.
Expression of cefF significantly decreased deacetoxycephalosporin C formation during cephalosporin C production in Acremonium chrysogenum
J. Ind. Microbiol. Biotechnol.
39
269-274
2012
Acremonium chrysogenum (P11935), Acremonium chrysogenum, Streptomyces clavuligerus (P42220), Streptomyces clavuligerus, Acremonium chrysogenum CGMCC3.3795 (P11935), Streptomyces clavuligerus CGMCC4.1611 (P42220)
Manually annotated by BRENDA team
Balakrishnan, N.; Ganesan, S.; Rajasekaran, P.; Rajendran, L.; Teddu, S.; Durairaaj, M.
Modified deacetylcephalosporin C synthase for the biotransformation of semisynthetic cephalosporins
Appl. Environ. Microbiol.
82
3711-3720
2016
Streptomyces clavuligerus (P42220), Streptomyces clavuligerus, Streptomyces clavuligerus ATCC 27064 (P42220)
Manually annotated by BRENDA team