1.14.11.21: clavaminate synthase
This is an abbreviated version!
For detailed information about clavaminate synthase, go to the full flat file.
Word Map on EC 1.14.11.21
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1.14.11.21
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clavulanic
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clavuligerus
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alpha-ketoglutarate
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proclavaminic
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beta-lactamase
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dioxygenase
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non-heme
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cs2
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oxygenases
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clavams
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dioxygen
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alpha-ketoglutarate-dependent
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desaturation
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amidinohydrolase
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2-his-1-carboxylate
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synthesis
- 1.14.11.21
-
clavulanic
- clavuligerus
- alpha-ketoglutarate
-
proclavaminic
- beta-lactamase
- dioxygenase
-
non-heme
- cs2
- oxygenases
-
clavams
- dioxygen
-
alpha-ketoglutarate-dependent
-
desaturation
-
amidinohydrolase
-
2-his-1-carboxylate
- synthesis
Reaction
Synonyms
CAS, CAS1, CAS2, clavaminate synthase 1, clavaminate synthase 2, clavaminic acid synthase, CS, synthase, clavaminate
ECTree
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Crystallization
Crystallization on EC 1.14.11.21 - clavaminate synthase
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anaerobically, recombinant isozyme CAS1, in complex with Fe2+, 2-oxoglutarate and deoxyguanidinoproclavaminate, hanging drop vapour diffusion method, protein solution: 20 mg/ml, reservoir solution: 0.1 M Tris, pH 7.5, 2 M ammonium sulfate, 10% glycerol, 20°C, treatment with NO under anaerobic conditions, structure determination and analysis
recombinant isozyme CAS1, hanging drop method, 2 M ammonium sulfate, 0.1 M Tris, 21°C, apoform crystals or in complex with Fe2+, N-alpha-acetyl-L-arginine, and the substrates 2-oxoglutarate and proclavaminate, X-ray diffraction structure determination for native and heavy atom derivatives and analysis