Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.11.18: phytanoyl-CoA dioxygenase

This is an abbreviated version!
For detailed information about phytanoyl-CoA dioxygenase, go to the full flat file.

Word Map on EC 1.14.11.18

Reaction

phytanoyl-CoA
+
2-oxoglutarate
 +
O2
=
2-hydroxyphytanoyl-CoA
 +
succinate
 +
CO2

Synonyms

PAHX, PhyH, PHYHD1, Phytanic acid oxidase, phytanoyl-CoA 2-hydroxylase, Phytanoyl-CoA alpha-hydroxylase, phytanoyl-CoA dioxygenase domain containing protein 1, phytanoyl-CoA hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.18 phytanoyl-CoA dioxygenase

Engineering

Engineering on EC 1.14.11.18 - phytanoyl-CoA dioxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D177A
E197Q
disruption of the 2-oxoglutarate binding pocket
F275S
places a polar side chain in a hydrophobic pocket and possibly interferes with the overall structure or impair protein folding
G204S
H175A
H213A
insoluble mutant enzyme
H259A
mutation removes two of the hydrogen bonds and probably destabilizes the beta-turn, which in turn destabilizes the core double stranded beta-helix
H264A
activity with phytanoyl-CoA is 7.5 of the wild-type activity
I199F
disruption of the 2-oxoglutarate binding pocket
N269H
N83Y
disruption of protein-protein interactions proposed to involve PAHX, such as that with sterol carrier protein-2, proposed to be responsible for solubilization and presentation of phytanoyl-CoA to PAHX
P29S
clinically observed mutant is fully active, mutation may result in a defective targeting of the protein to peroxisomes
Q176A
activity with phytanoyl-CoA is 16.3% of the wild-type activity
Q176K
mutation causes partial uncoupling of 2-oxoglutarate conversion from phytanoyl-CoA oxidation
R245Q
disruption of protein-protein interactions proposed to involve PAHX, such as that with sterol carrier protein-2, proposed to be responsible for solubilization and presentation of phytanoyl-CoA to PAHX
R275Q
R275W
W193R
disruption of the 2-oxoglutarate binding pocket