for the NTL8 complementation test, a sequence containing the NTL8 gene with its promoter is subcloned into pKGWFS7 Gateway vector, for histochemical staining an NTL8 promoter region is transcriptionally fused to the GUS-coding sequence, the fusion product transformed into Arabidopsis
the anti gibberellin 24 single monoclonal antibody gene (anti-GA24 scFv) is PCR-amplified with plasmid pH1L24B and introduced into the binary vector pBI-ER-D, transferred into Agrobacterium tumefaciens strain LBA4404 to transform Arabidospsis thaliana, the antibody is fused with the green fluorescence protein (GFP), a c-myc epitope tag and KDEL ER-retention signal
transgenic plants of the liliaceous ornamental Tricyrtis sp. Shinonome overexpressing the GA20ox or GA3ox gene from Torenia fournieri (TfGA20ox2 and TfGA3ox1) aree produced. After 3 years of cultivation, 4 and 2 independent transgenic plants containing TfGA20ox2 and TfGA3ox1, respectively, are subjected to morphological characterization at the flowering stage. Because GA20ox and GA3ox catalyze the last step in the formation of bioactive gibberellins, overexpression of TfGA20ox2 or TfGA3ox1 is initially expected to induce a gibberellin-overproduction phenotype in transgenic plants, such as internode elongation. However, on the contrary, all the transgenic plants exhibit reduced plant height, reduced internode length and reduced stem diameter compared with the control, non-transgenic plants, irrespective of the kind of transgene. In addition, all the transgenic plants have slender leaves and narrow flower tepals. Exogenous treatment of transgenic plants with gibberellic acid and a GA biosynthesis inhibitor, uniconazol, result in increased and decreased plant height, respectively