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1.14.11.11: hyoscyamine (6S)-dioxygenase

This is an abbreviated version!
For detailed information about hyoscyamine (6S)-dioxygenase, go to the full flat file.

Word Map on EC 1.14.11.11

Reaction

L-hyoscyamine
+
2-oxoglutarate
 +
O2
=
(6S)-hydroxyhyoscyamine
 +
succinate
 +
CO2

Synonyms

6H6, AaH6H, AtH6H, Dih6h, H6H, HnH6H, hyoscyamine 6-hydroxylase, hyoscyamine 6beta-hydroxylase, hyoscyamine-6beta-hydroxylase, oxygenase, hyoscyamine 6beta-di-

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.11 hyoscyamine (6S)-dioxygenase

Application

Application on EC 1.14.11.11 - hyoscyamine (6S)-dioxygenase

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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
the enzyme is a molecular marker for PCR-based screening approach for tropane alkaloids producing endophytic fungi
drug development
tropane alkaloids, such as hyoscyamine, 6-hydroxyhyoscyamine and scopolamine, are secondary metabolites that are traditionally applied in medicine due to their anticholinergic activity. An alternative strategy for the production of the most valuable alkaloids, 6beta-hydroxyhyoscyamine and scopolamine, using Escherichia coli harboring the hyoscyamine-6beta-hydroxylase enzyme as biocatalysts is developed
pharmacology
tropane alkaloids including hyoscyamine, anisodamine, scopolamine and anisodine, are used medicinally as anticholinergic agents with increasing market demand, improvement of production by metabolic engineering introduction of genes encoding the branch-controlling enzyme tropinone reductase I and the downstream rate-limiting enzyme hyoscyamine-6beta-hydroxylase
synthesis
overexpression of the enzyme (H6H) enhances the biosynthesis of anisodamine and scopolamine in hairy root cultures of Scopolia lurida, which should improve its commercial value. The tropane alkaloids anisodamine and and scopolamine are clinically used as anticholinergic agents