1.13.99.1: inositol oxygenase
This is an abbreviated version!
For detailed information about inositol oxygenase, go to the full flat file.
Word Map on EC 1.13.99.1
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1.13.99.1
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d-glucuronate
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glucaric
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diiron
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ambience
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mixed-valent
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four-electron
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glucuronate-xylulose
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medicine
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diagnostics
- 1.13.99.1
- d-glucuronate
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glucaric
-
diiron
-
ambience
-
mixed-valent
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four-electron
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glucuronate-xylulose
- medicine
- diagnostics
Reaction
Synonyms
amyoinositol oxygenase, AtMIOX, EC 1.13.1.11, EC 1.99.2.6, GsMIOX1a, Inositol oxygenase, inositol oxygenase 1, InOx, Kidney-specific protein 32, meso-Inositol oxygenase, MIOX, MIOX1, MIOX2, MIOX4, MIOX5, mMIOX, MOO, Myo-inositol oxygenase, OsMIOX, Oxygenase, inositol, ppMIOX, Renal-specific oxidoreductase, renal-specific oxidoreductase/myo-inositol oxygenase, RSOR/MIOX
ECTree
1.13.99.1 inositol oxygenaseAdvanced search results
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results (Engineering
Engineering on EC 1.13.99.1 - inositol oxygenase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
DELTA miox 1+2
double mutant, smaller syncytia, less female nematodes per plant
delta miox 4+5
double mutant, smaller syncytia, less female nematodes per plant
DELTA miox1
single knockout mutant of one allele of MIOX
delta miox2
single knockout mutant of one allele of MIOX
delta miox5
single knockout mutant of one allele of MIOX
D82Y
the mutant displays significantly decreased Km values and better kcat value compared to the wild type enzyme
L240M
the mutant's activity shows a drastic improvement compared with the wild type enzyme
Q62H
the mutant displays significantly decreased Km values and lower kcat value compared to the wild type enzyme
Q62H/S173N/L240M
the mutant's activity shows a drastic improvement compared with the wild type enzyme
S173N
the mutant displays significantly decreased Km values and better kcat value compared to the wild type enzyme
S24A/S205A/S218A/T29A/T69A
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mutated putative PKA phosphorylation sites: mutant is also phosphorylated but to a minor degree
S24A/S205A/S218A/T29A/T69A/S64A/T40A/T49A/T69A/T253A
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mutated putative PKA and PKC phosphorylation sites: mutant is also phosphorylated but to a minor degree
S64A/T40A/T49A/T69A/T253A
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mutated putative PKC phosphorylation sites: mutant is also phosphorylated but to a minor degree
additional information
additional information
construction of a quadruple (miox1/2/4/5) mutant by mutation of all four MIOX isozymes via T-DNA insertion, metabolite profiling of syncytia induced in roots of the myo-inositol oxygenase quadruple mutant, overview. The quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. The miox1/2/4/5 plants show a similar reduction in the number of females to miox double mutants
additional information
construction of a quadruple (miox1/2/4/5) mutant by mutation of all four MIOX isozymes via T-DNA insertion, metabolite profiling of syncytia induced in roots of the myo-inositol oxygenase quadruple mutant, overview. The quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. The miox1/2/4/5 plants show a similar reduction in the number of females to miox double mutants
additional information
construction of a quadruple (miox1/2/4/5) mutant by mutation of all four MIOX isozymes via T-DNA insertion, metabolite profiling of syncytia induced in roots of the myo-inositol oxygenase quadruple mutant, overview. The quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. The miox1/2/4/5 plants show a similar reduction in the number of females to miox double mutants
additional information
construction of a quadruple (miox1/2/4/5) mutant by mutation of all four MIOX isozymes via T-DNA insertion, metabolite profiling of syncytia induced in roots of the myo-inositol oxygenase quadruple mutant, overview. The quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. The miox1/2/4/5 plants show a similar reduction in the number of females to miox double mutants
additional information
generation of an AtMIOX1 gene T-DNA insertion mutant atmiox1, the AtMIOX1 gene is efficiently silenced by the T-DNA insertion in the atmiox1 mutant. Constitutive overepression of Glycine soja MIOX1 under constitutive CaMV35S promoter control can recover the phenotype leading to alkaline stress tolerance in the atmiox1 mutant plants, overview
additional information
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construction of a quadruple (miox1/2/4/5) mutant by mutation of all four MIOX isozymes via T-DNA insertion, metabolite profiling of syncytia induced in roots of the myo-inositol oxygenase quadruple mutant, overview. The quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. The miox1/2/4/5 plants show a similar reduction in the number of females to miox double mutants
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additional information
coexpression of the Mus musculus mMIOX and the urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 as fusion protein leads highly increased glutaric acid levels from myo-inositol in transfected Pichia pastoris cells. Production of glucaric acid with a fed-batch fermentation strategy, overview. Recombinant coexpression of the enzyme in Escherichia coli strains BL21(DE3) or MG1655 with myo-inositol-1-phosphate synthase from Saccharomyces cerevisiae and urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 leads to highly increased glutaric acid production, especially with addition of myo-inositol instead of glucose, production parameters, overview
additional information
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coexpression of the Mus musculus mMIOX and the urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 as fusion protein leads highly increased glutaric acid levels from myo-inositol in transfected Pichia pastoris cells. Production of glucaric acid with a fed-batch fermentation strategy, overview. Recombinant coexpression of the enzyme in Escherichia coli strains BL21(DE3) or MG1655 with myo-inositol-1-phosphate synthase from Saccharomyces cerevisiae and urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 leads to highly increased glutaric acid production, especially with addition of myo-inositol instead of glucose, production parameters, overview
additional information
under high-glucose ambience, MIOX overexpression accentuates redox imbalance, perturbed NAD+/NADH ratios, increased ROS generation, depleted reduced glutathione, reduced GSH/GSSG ratio, and enhanced adaptive changes in the profile of the antioxidant defense system. These changes are also accompanied by mitochondrial dysfunctions, DNA damage and induction of apoptosis, accentuated activity of profibrogenic cytokine, and expression of fibronectin, the latter two being the major hallmarks of diabetic nephropathy. These perturbations are largely blocked by various reactive oxygen species inhibitors (Mito Q, diphenyleneiodonium chloride, and N-acetylcysteine) and MIOX/NOX4 siRNA, overview. Cells under high glucose ambience or transfected with MIOX-pcDNA show increased expression of apoptogenic protein Bax. The expression is further increased following concomitant treatment with HG and MIOX transfection. Treatment with N-acetylcysteine reduces the expression of Bax
