1.13.12.9: phenylalanine 2-monooxygenase

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For detailed information about phenylalanine 2-monooxygenase, go to the full flat file.

Reaction

Synonyms

L-Phe oxidase, L-phenylalanine oxidase, L-phenylalanine oxidase (deaminating and decarboxylating), oxidase, phenylalanine (deaminating, decarboxylating), PAOpt, proPAO

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

                1.13.12.9 phenylalanine 2-monooxygenase

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Results	in table
2	Activating Compound
13	AA Sequence
2	Application
3	CAS Registry Number
3	Cloned(Commentary)
5	Cofactor
3	Crystallization (Commentary)
14	Inhibitors
6	Ki Value [mM]
20	KM Value [mM]
2	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
78	Organism
2	Pathway
12	PDB ID
3	pH Optimum
1	pH Range
17	Protein Variants
4	Purification (Commentary)
3	Reaction
5	Reaction Type
20	Reference
1	Specific Activity [micromol/min/mg]
1	Storage Stability
61	Substrates and Products (Substrate)
4	Subunits
17	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
2	Temperature Range [°C]
3	Temperature Stability [°C]
2	Turnover Number [1/s]

Purification

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Purification on EC 1.13.12.9 - phenylalanine 2-monooxygenase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

2 entries

cell disruption by sonication, centrifugation, supernatant applied to HisTrap HP column equilibrated with 20 mM Tris-HCl (pH 8.0) containing 500 mM NaCl, elution with linear gradient of imidazole from 0-0.5 M, ammonium sulfate added to eluted fractions, centrifugation of precipitate, solution in 20 mM Tris-HCl (pH 8.0) containing 300 mM ammonium sulfate, several chromatographic steps using HiTrap Phenyl FF, Resource Q, and HiLoad 16/60 Superdex 200 prep grade, activated enzyme purified by HiTrap Q HP and gel filtration

Pseudomonas sp.

-

698757

recombinant enzyme using His-tag

Pseudomonas sp.

Q5W9R9

659094

-

Pseudomonas sp.

-

439317

-

Pseudomonas sp.

Q5W9R9

727793