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1.13.12.7: firefly luciferase

This is an abbreviated version!
For detailed information about firefly luciferase, go to the full flat file.

Word Map on EC 1.13.12.7

Reaction

D-firefly luciferin
+
O2
+
ATP
=
firefly oxyluciferin
+
CO2
+
AMP
+
diphosphate
+
hnu

Synonyms

AL1, AL2, beetle luciferase, CBG99luc, CBRluc, FFL, firefly luciferase, firefly luciferin luciferase, fluc, LpLuc1, LpLuc2, Luc, Luc1, Luc1-type luciferase, Luc2, Luc2-type luciferase, luciferase, luciferase (firefly luciferin), luciferase FM, luciferin, Luciola italica luciferase, lucPpe, lucPpy, orange light-producing luciferase, oxygen 4-oxidoreductase, PC3-Luc, Photinus luciferin 4-monooxygenase (ATP-hydrolyzing), Photinus pyralis luciferase, PML, PpLase, Ppy, Ppy GR-TS, Ppy RE-TS, PpyWT, PsntWT

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.7 firefly luciferase

Substrates Products

Substrates Products on EC 1.13.12.7 - firefly luciferase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(4S)-2-(6-hydroxy-1,3-benzoxazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid + ATP + O2
?
show the reaction diagram
-
decay time to 10%: 500 sec, relative specific activity: 13%
-
-
?
(4S)-2-(6-hydroxy-1-benzofuran-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid + ATP + O2
?
show the reaction diagram
-
decay time to 10%: 150 sec, relative specific activity: 1.7%
-
-
?
(4S)-2-(6-hydroxy-1-benzothiophen-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid + ATP + O2
?
show the reaction diagram
-
decay time to 10%: 5700 sec, relative specific activity: 421%
-
-
?
(4S)-2-(6-hydroxy-1H-benzimidazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid + ATP + O2
?
show the reaction diagram
-
decay time to 10%: 400 sec, relative specific activity: 0.4%
-
-
?
(4S)-2-(6-hydroxy-1H-indol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid + ATP + O2
?
show the reaction diagram
-
-
-
-
?
4'-methyl-D-luciferin + ATP + O2
?
show the reaction diagram
-
-
-
?
5,5'-dimethylluciferin + ATP + O2
dehydroluciferin-adenylate + diphosphate
show the reaction diagram
-
produces only red light, 6-amino and 4 hydroxy analogs tested
-
?
5,5-dimethyl-luciferyl-O-adenosine monophosphate + ?
5,5-dimethyloxyluciferin + hv
show the reaction diagram
5,5-dimethyl-luciferyl-O-adenosine monophosphate + ?
5,5-dimethyloxyluciferin + hv + ?
show the reaction diagram
-
-
-
-
?
5,5-dimethylluciferin + ATP
5,5-dimethyloxyluciferin + CO2 + H2O + AMP + diphosphate + hv
show the reaction diagram
-
-
-
-
?
5-methylluciferin + ATP
5-methyloxyluciferin + CO2 + H2O + AMP + diphosphate + hv
show the reaction diagram
-
-
-
-
?
5-methylluciferin + ATP + O2
?
show the reaction diagram
-
-
-
?
5-monomethyl-luciferyl-O-adenosine monophosphate + ?
5-monomethyloxyluciferin + hv + ?
show the reaction diagram
-
-
-
-
?
6'-amino-D-luciferin + ATP
? + hv
show the reaction diagram
-
-
-
-
?
6'-amino-D-luciferin + ATP + O2
?
show the reaction diagram
-
-
-
?
8-anilino-1-naphthalene-sulfonate + ?
?
show the reaction diagram
-
-
-
?
adenosine 5'-tetraphosphate + Photinus luciferin
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
show the reaction diagram
-
0.75% light response relative to ATP
-
?
ADP
ATP + AMP
show the reaction diagram
-
-
reaction inhibited by P1,P5-di(adenosine-5'-)pentaphosphate, but independent of luciferin
?
arachidonic acid + ATP + CoA
arachidonoyl-CoA + ?
show the reaction diagram
-
-
-
-
?
arachidonic acid + ATP + CoA
arachidonoyl-CoA + AMP + diphosphate
show the reaction diagram
-
-
-
-
?
ATP + O2 + oxyluciferin
?
show the reaction diagram
-
assay at pH 7.5, 24-27°C
-
-
?
coelenterazine disulfate + ATP + O2 + H2O
coelenteramide disulfate + AMP + CO2 + diphosphate
show the reaction diagram
-
-
-
?
D-5,5-dimethylluciferyl-adenylate + O2
?
show the reaction diagram
yellow-green color of luminescence
-
-
?
D-5,5-dimethylluciferyl-adenylate + O2 + ATP
?
show the reaction diagram
red color of luminescence
-
-
?
D-aminoluciferin + ATP + O2
?
show the reaction diagram
-
assay at 37°C. 5 min
-
-
?
D-firefly luciferin + O2 + ATP
firefly oxyluciferin + CO2 + AMP + diphosphate + hv
show the reaction diagram
D-luciferin + ATP
diphosphate + luciferyl-adenylate
show the reaction diagram
-
-
-
r
D-luciferin + ATP + O2
?
show the reaction diagram
D-luciferin + ATP + O2
luciferyl-adenylate + diphosphate
show the reaction diagram
D-luciferin + ATP + O2
luciferyl-adenylate + diphosphate + H2O + hv
show the reaction diagram
D-luciferin + ATP + O2
luciferyl-AMP + diphosphate
show the reaction diagram
-
assay at pH 7.8
-
-
r
D-luciferin + ATP + O2
oxidized D-luciferin + AMP + CO2 + diphosphate
show the reaction diagram
D-luciferin + ATP + O2
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
show the reaction diagram
D-luciferin + ATP + O2
oxidized luciferin + AMP + CO2 + diphosphate
show the reaction diagram
-
-
-
-
?
D-luciferin + ATP + O2
oxidized luciferin + AMP + CO2 + diphosphate + hv
show the reaction diagram
D-luciferin + ATP + O2
oxyluciferin + CO2 + AMP + hv + diphosphate
show the reaction diagram
-
-
-
-
?
D-luciferin + dATP + O2
?
show the reaction diagram
-
-
-
-
?
D-luciferin + O2
?
show the reaction diagram
-
10 microM, assay at pH 7.5
-
-
?
D-luciferin + O2 + ATP
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
show the reaction diagram
D-luciferin + O2 + ATP
oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
show the reaction diagram
D-luciferin + O2 + ATP
oxyluciferin + AMP + diphosphate + CO2 + light
show the reaction diagram
-
-
-
?
D-luciferin + O2 + ATP
oxyluciferin + CO2 + H2O + AMP + diphosphate + hv
show the reaction diagram
-
-
-
-
r
D-luciferyl-adenylate + O2
oxyluciferin + CO2 + AMP + hv
show the reaction diagram
-
-
-
ir
D-luciferyl-AMP + O2
dehydroluciferyl-AMP + H2O2
show the reaction diagram
-
-
-
-
ir
D-luciferyl-O-adenosine monophosphate + ?
?
show the reaction diagram
-
-
-
?
D-naphthylluciferin + ATP + O2
?
show the reaction diagram
-
-
-
?
D-quinolylluciferin + ATP + O2
?
show the reaction diagram
-
-
-
?
decanoic acid + ATP + CoA
decanoyl-CoA + AMP + diphosphate
show the reaction diagram
dehydroluciferin + ATP
dehydroluciferyl-AMP + diphosphate
show the reaction diagram
dehydroluciferin + ATP + O2
diphosphate + dehydroluciferin-adenylate
show the reaction diagram
-
-
-
?
dehydroluciferin + CoA
dehydroluciferyl-CoA
show the reaction diagram
-
-
-
-
?
dehydroluciferyladenylate + CoA
dehydroluciferyl-CoA + ?
show the reaction diagram
-
-
-
-
?
deoxyATP + Photinus luciferin
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
show the reaction diagram
-
-
-
?
fatty acid + ATP + CoA
fatty acyl-CoA + AMP
show the reaction diagram
-
-
-
?
L-luciferin + CoA + ATP
luciferyl-CoA + AMP + diphosphate
show the reaction diagram
-
L-luciferin is converted to luciferyl adenylate, and the adenyl group of luciferyl adenylate is then substituted to CoA-SH to give luciferyl-CoA. Even in presence of CoA-SH, D-luciferin is used for the light production reaction, but is not converted into luciferyl-CoA
-
-
?
lauric acid + ATP + CoA
lauroyl-CoA + AMP + diphosphate
show the reaction diagram
linoleic acid + ATP + CoA
linoleoyl-CoA + AMP + diphosphate
show the reaction diagram
linolenic acid + ATP + CoA
linolenoyl-CoA + AMP + diphosphate
show the reaction diagram
luciferin + ATP + O2
?
show the reaction diagram
-
assay at 25°C, pH 7.8
-
-
?
luciferin + ATP + O2
oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
show the reaction diagram
luciferin + ATP + O2
oxyluciferin + AMP + CO2 + diphosphate
show the reaction diagram
-
-
-
-
?
luciferin + MgATP2-
AMP + diphosphate
show the reaction diagram
luciferin + O2 + ATP
?
show the reaction diagram
-
assay at pH 7.8
-
-
?
luciferin + O2 + ATP
oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
show the reaction diagram
luciferin + O2 + ATP
oxyluciferin + AMP + diphosphate + CO2 + light
show the reaction diagram
-
-
-
-
ir
luciferyl adenylate + O2
dehydroluciferyl adenylate + ?
show the reaction diagram
-
-
-
-
?
luciferyl-adenylate
oxyluciferin + hnu
show the reaction diagram
-
-
-
?
luciferyl-adenylate + O2
oxyluciferin + AMP + CO2 + hv
show the reaction diagram
luciferyl-AMP + O2
oxyluciferin + hv + CO2 + AMP + diphosphate
show the reaction diagram
-
-
-
-
r
luciferyl-O-adenosine monophosphate + ?
?
show the reaction diagram
luciferyl-O-adenosine monophosphate + CoA
luciferyl-CoA + AMP
show the reaction diagram
-
-
-
?
myristic acid + ATP + CoA
myristoyl-CoA + AMP + diphosphate
show the reaction diagram
oleic acid + ATP + CoA
oleoyl-CoA + AMP + diphosphate
show the reaction diagram
P1,P5-di(adenosine-5'-)pentaphosphate + Photinus luciferin
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
show the reaction diagram
-
2.2% light response relative to ATP
-
?
palmitic acid + ATP + CoA
palmitoyl-CoA + AMP + diphosphate
show the reaction diagram
-
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
show the reaction diagram
Photinus luciferin + O2 + GTP
adenosine 5'-P1-tetraphospho-P4-5'''-guanosine
show the reaction diagram
-
-
-
?
polyethylene glycol-6-amino-D-luciferin
? + hv
show the reaction diagram
-
longer light emission
-
-
?
R-COOH + ATP
R-CO-AMP + diphosphate
show the reaction diagram
additional information
?
-