1.13.12.7: firefly luciferase
This is an abbreviated version!
For detailed information about firefly luciferase, go to the full flat file.
Word Map on EC 1.13.12.7
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1.13.12.7
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bioluminescence
-
luminescence
-
chemiluminescence
-
emit
-
renilla
-
noninvasive
-
luciferases
-
cypridina
-
photon
-
lentiviral
-
adenovirus
-
click
-
luciferase-expressing
-
herpes
-
engraft
-
nude
-
co-transfected
-
cytomegalovirus
-
camera
-
electroporation
-
simplex
-
hsp70
-
emitter
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replication-deficient
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excited-state
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promoter-driven
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charge-coupled
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nonviral
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nanoluc
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non-invasively
-
gaussia
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luminol
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intramyocardial
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coelenterazine
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bicistronic
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yellow-green
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molecular biology
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light-emitting
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cap-independent
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aequorin
-
biotechnology
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red-shifted
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dnaj
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luminometer
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grpe
-
polyethylenimine
-
bioimaging
-
luciferase-based
-
medicine
-
photoproteins
-
glow
-
analysis
-
multicolor
-
brighter
- 1.13.12.7
-
bioluminescence
-
luminescence
-
chemiluminescence
-
emit
- renilla
-
noninvasive
- luciferases
- cypridina
-
photon
-
lentiviral
- adenovirus
-
click
-
luciferase-expressing
-
herpes
-
engraft
-
nude
-
co-transfected
- cytomegalovirus
-
camera
-
electroporation
- simplex
- hsp70
-
emitter
-
replication-deficient
-
excited-state
-
promoter-driven
-
charge-coupled
-
nonviral
- nanoluc
-
non-invasively
- gaussia
- luminol
-
intramyocardial
- coelenterazine
-
bicistronic
-
yellow-green
- molecular biology
-
light-emitting
-
cap-independent
- aequorin
- biotechnology
-
red-shifted
- dnaj
-
luminometer
- grpe
- polyethylenimine
-
bioimaging
-
luciferase-based
- medicine
-
photoproteins
-
glow
- analysis
-
multicolor
-
brighter
Reaction
Synonyms
AL1, AL2, beetle luciferase, CBG99luc, CBRluc, FFL, firefly luciferase, firefly luciferin luciferase, fluc, LpLuc1, LpLuc2, Luc, Luc1, Luc1-type luciferase, Luc2, Luc2-type luciferase, luciferase, luciferase (firefly luciferin), luciferase FM, luciferin, Luciola italica luciferase, lucPpe, lucPpy, orange light-producing luciferase, oxygen 4-oxidoreductase, PC3-Luc, Photinus luciferin 4-monooxygenase (ATP-hydrolyzing), Photinus pyralis luciferase, PML, PpLase, Ppy, Ppy GR-TS, Ppy RE-TS, PpyWT, PsntWT
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Metals Ions
Metals Ions on EC 1.13.12.7 - firefly luciferase
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Cu2+
Mg2+
Zn2+
additional information
Cu2+
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promotes the binding to Cibacron brilliant blue and Cibacron blue F3GA
Mg2+
the enzyme catalyzes the oxidation of substrate luciferin in the presence of Mg2+ (10 mM)
Mg2+
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optimal concentration for wild-type enzyme and N-terminal domain: 10 mM
Mg2+
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enzyme activity without 10 mM MgSO4 decreases to about 50% of that of wild type enzyme in the presence of Mg2+
Mg2+
the enzyme catalyzes the oxidation of substrate luciferin in the presence of Mg2+ (10 mM)
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increasing concentration shifts the light wavelenghts to higher values
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Mg2+, Ca2+, Zn2+, Ni2+, Cu2+, Fe3+, Li+, Na+, K+ not required for activity
additional information
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activity in decreasing order: Mn2+, Mg2+, Co2+/Zn2+/Fe2+, Cd2+, Ni2+, Ca2+, Sr2+