1.13.12.7: firefly luciferase
This is an abbreviated version!
For detailed information about firefly luciferase, go to the full flat file.
Word Map on EC 1.13.12.7
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1.13.12.7
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bioluminescence
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luminescence
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chemiluminescence
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emit
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renilla
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noninvasive
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luciferases
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cypridina
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photon
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lentiviral
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adenovirus
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click
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luciferase-expressing
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herpes
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engraft
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nude
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co-transfected
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cytomegalovirus
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camera
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electroporation
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simplex
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hsp70
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emitter
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replication-deficient
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excited-state
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promoter-driven
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charge-coupled
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nonviral
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nanoluc
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non-invasively
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gaussia
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luminol
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intramyocardial
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coelenterazine
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bicistronic
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yellow-green
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molecular biology
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light-emitting
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cap-independent
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aequorin
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biotechnology
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red-shifted
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dnaj
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luminometer
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grpe
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polyethylenimine
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bioimaging
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luciferase-based
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medicine
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photoproteins
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glow
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analysis
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multicolor
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brighter
- 1.13.12.7
-
bioluminescence
-
luminescence
-
chemiluminescence
-
emit
- renilla
-
noninvasive
- luciferases
- cypridina
-
photon
-
lentiviral
- adenovirus
-
click
-
luciferase-expressing
-
herpes
-
engraft
-
nude
-
co-transfected
- cytomegalovirus
-
camera
-
electroporation
- simplex
- hsp70
-
emitter
-
replication-deficient
-
excited-state
-
promoter-driven
-
charge-coupled
-
nonviral
- nanoluc
-
non-invasively
- gaussia
- luminol
-
intramyocardial
- coelenterazine
-
bicistronic
-
yellow-green
- molecular biology
-
light-emitting
-
cap-independent
- aequorin
- biotechnology
-
red-shifted
- dnaj
-
luminometer
- grpe
- polyethylenimine
-
bioimaging
-
luciferase-based
- medicine
-
photoproteins
-
glow
- analysis
-
multicolor
-
brighter
Reaction
Synonyms
AL1, AL2, beetle luciferase, CBG99luc, CBRluc, FFL, firefly luciferase, firefly luciferin luciferase, fluc, LpLuc1, LpLuc2, Luc, Luc1, Luc1-type luciferase, Luc2, Luc2-type luciferase, luciferase, luciferase (firefly luciferin), luciferase FM, luciferin, Luciola italica luciferase, lucPpe, lucPpy, orange light-producing luciferase, oxygen 4-oxidoreductase, PC3-Luc, Photinus luciferin 4-monooxygenase (ATP-hydrolyzing), Photinus pyralis luciferase, PML, PpLase, Ppy, Ppy GR-TS, Ppy RE-TS, PpyWT, PsntWT
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Cloned
Cloned on EC 1.13.12.7 - firefly luciferase
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a chimeric protein derived from Photinus pyralis and Luciola cruciata luciferases expressed in Escherichia coli
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cloning of the cDNA encoding the destabilized enzyme into an adenoviral expression plasmid and transfection of Vero, Hep-2, Chang, A-549, COS-1, and HeLa cells, luciferase expression is linear with respect to viral multiplicity of infection, protein synthesis inhibiting drugs, e.g. shiga toxins of Escherichia coli, diphtheria toxin, Pseudomonas exotoxin A and the plant toxin ricin A, and cycloheximide, reduce bioluminescence respresenting the antiviral activity
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expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli JM109 cells and Bacillus subtilis strain NBRC13719
expressed in Escherichia coli strain BL21
expressed in Escherichia coli strains XL1-Blue and BL21
expression in Escherichia coli
expression in Escherichia coli BL21
expression in Escherichia coli BL21 and DH5alpha, transfection in CHO-cells
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expression in Escherichia coli, transfection in human glioma cells and in quadriceps muscles of mice
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expression in Spodoptera frugiperda clone 9-cells, expression in insect cells using a baculovirus vector
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expression in transfected Leishmania amazonensis strain LV79 amastigotes, transfection by electroporation
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expression of a recombinant enzyme containing a protein kinase A recognition site
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expression of a recombinant luciferase-ubiquitin enzyme in Saccharomyces cerevisiae
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gene expression in vegetative and symbiotic Rhizobium melioti and other gram-negative bacteria
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gene luc, DNA and amino acid sequence determination and analysis, sequence comparisons of Photinus pyralis and Photinus scintillans luciferases, the single conservative amino acid change tyrosine to phenylalanine at position 255 accounts for the entire emission color difference, recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) pLysS, recombinant Luc contains the N-terminal peptide GlyProLeuGlySer-
mutant enzymes,R218A, H245A, G246A, F247A, F247L, F247Y, F250G, F250S, T251A, G315A, G316A, G341A, L342A, T343A, S347A, A348V, I351A and K529A
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recombinant coexpression of the luciferase with fused cyclization proteins SpyCatcher at the N-terminus and SpyTag at the C-terminus (pyCatcher-LUC-SpyTag) in Escherichia coli strain BL21(DE3)
recombinant expression from vector p35SHSPG in Nicotiana benthamiana BY-2 cells with intracellular enzyme localization, recombinant expression of the enzyme in Arabidopsis thaliana roots with enzyme localization around the nucleus, outside of the central vacuole and in the protoplasm
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
recombinant expression of the chimeric mutant luc2 that contains the N-domain of Photinus pyralis luciferase joined to the C-domain of Luciola italica luciferase in HEK-293 cells and in Escherichia coli strain BL21(DE3)pLysS
wild-type and mutant enzymes are expressed as GST-fusion proteins
wild-type and mutant enzymes expressed as glutathione-S-transferase-fusion proteins containing the additional N-terminal peptide Gly-Pro-Leu-Gly-SER, which remains after PreScission protease cleavage from GST, expression in Escherichia coli
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gene luc, DNA and amino acid sequence determination and analysis, sequence comparisons of Photinus pyralis and Photinus scintillans luciferases, the single conservative amino acid change tyrosine to phenylalanine at position 255 accounts for the entire emission color difference, recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) pLysS, recombinant Luc contains the N-terminal peptide GlyProLeuGlySer-
gene luc, DNA and amino acid sequence determination and analysis, sequence comparisons of Photinus pyralis and Photinus scintillans luciferases, the single conservative amino acid change tyrosine to phenylalanine at position 255 accounts for the entire emission color difference, recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) pLysS, recombinant Luc contains the N-terminal peptide GlyProLeuGlySer-
recombinant expression of the chimeric mutant luc2 that contains the N-domain of Photinus pyralis luciferase joined to the C-domain of Luciola italica luciferase in HEK-293 cells and in Escherichia coli strain BL21(DE3)pLysS
wild-type and mutant enzymes are expressed as GST-fusion proteins
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