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1.13.12.7: firefly luciferase

This is an abbreviated version!
For detailed information about firefly luciferase, go to the full flat file.

Word Map on EC 1.13.12.7

Reaction

D-firefly luciferin
+
O2
 +
ATP
=
firefly oxyluciferin
 +
CO2
 +
AMP
 +
diphosphate
 +
hnu

Synonyms

AL1, AL2, beetle luciferase, CBG99luc, CBRluc, FFL, firefly luciferase, firefly luciferin luciferase, fluc, LpLuc1, LpLuc2, Luc, Luc1, Luc1-type luciferase, Luc2, Luc2-type luciferase, luciferase, luciferase (firefly luciferin), luciferase FM, luciferin, Luciola italica luciferase, lucPpe, lucPpy, orange light-producing luciferase, oxygen 4-oxidoreductase, PC3-Luc, Photinus luciferin 4-monooxygenase (ATP-hydrolyzing), Photinus pyralis luciferase, PML, PpLase, Ppy, Ppy GR-TS, Ppy RE-TS, PpyWT, PsntWT

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
                1.13.12.7 firefly luciferase

Cloned

Cloned on EC 1.13.12.7 - firefly luciferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a chimeric protein derived from Photinus pyralis and Luciola cruciata luciferases expressed in Escherichia coli
-
cloning of the cDNA encoding the destabilized enzyme into an adenoviral expression plasmid and transfection of Vero, Hep-2, Chang, A-549, COS-1, and HeLa cells, luciferase expression is linear with respect to viral multiplicity of infection, protein synthesis inhibiting drugs, e.g. shiga toxins of Escherichia coli, diphtheria toxin, Pseudomonas exotoxin A and the plant toxin ricin A, and cycloheximide, reduce bioluminescence respresenting the antiviral activity
-
complete nucleotide sequence, expression in mammalian cells
-
Escherichia coli BL21 (DE3)
expressed in Candida albicans strain CAI4
-
expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expressed in Escherichia coli JM109 cells and Bacillus subtilis strain NBRC13719
expressed in Escherichia coli strain BL21
expressed in Escherichia coli strain HMS174(DE3)
expressed in Escherichia coli strain JM109
expressed in Escherichia coli strain LE 392
-
expressed in Escherichia coli strains XL1-Blue and BL21
expressed in Escherichia coli Top10 cells
-
expressed in Escherichia coli XL1-Blue cells
expressed in Escherichia coli XL10-Gold cells
-
expressed in HepG2 cells
expressed in Saccharomyces cerevisiae
-
expression in Drosophila
-
expression in Escherichia coli
expression in Escherichia coli BL21
expression in Escherichia coli BL21 and DH5alpha, transfection in CHO-cells
-
expression in Escherichia coli BL21 and XL1blue
expression in Escherichia coli BL21 Star (DE3)
expression in Escherichia coli BL21-DE3 cells
Amydetes vivianii
expression in Escherichia coli, transfection in human glioma cells and in quadriceps muscles of mice
-
expression in HEK-293 cells or Grip-Tite 293 MSR cells
-
expression in HEK-293T cells
-
expression in L16 derived from CV-1 monkey kidney cells
-
expression in mouse cells
-
expression in PC3 cells
-
expression in Spodoptera frugiperda clone 9-cells, expression in insect cells using a baculovirus vector
-
expression in transfected Leishmania amazonensis strain LV79 amastigotes, transfection by electroporation
-
expression of a polyhystidine tagged enzyme in Trichoplusia
-
expression of a recombinant enzyme containing a protein kinase A recognition site
-
expression of a recombinant luciferase-ubiquitin enzyme in Saccharomyces cerevisiae
-
expression of the wild type and three mutants in Escherichia coli
-
firefly lantern cDNA library
-
gene expression in vegetative and symbiotic Rhizobium melioti and other gram-negative bacteria
-
gene luc, DNA and amino acid sequence determination and analysis, sequence comparisons of Photinus pyralis and Photinus scintillans luciferases, the single conservative amino acid change tyrosine to phenylalanine at position 255 accounts for the entire emission color difference, recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) pLysS, recombinant Luc contains the N-terminal peptide GlyProLeuGlySer-
into pET28a vector
mutant enzymes,R218A, H245A, G246A, F247A, F247L, F247Y, F250G, F250S, T251A, G315A, G316A, G341A, L342A, T343A, S347A, A348V, I351A and K529A
-
Nn-terminal domain, expression in Escherichia coli
-
overexpressed in Escherichia coli BL-21
-
recombinant coexpression of the luciferase with fused cyclization proteins SpyCatcher at the N-terminus and SpyTag at the C-terminus (pyCatcher-LUC-SpyTag) in Escherichia coli strain BL21(DE3)
recombinant expression from vector p35SHSPG in Nicotiana benthamiana BY-2 cells with intracellular enzyme localization, recombinant expression of the enzyme in Arabidopsis thaliana roots with enzyme localization around the nucleus, outside of the central vacuole and in the protoplasm
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
recombinant expression of the chimeric mutant luc2 that contains the N-domain of Photinus pyralis luciferase joined to the C-domain of Luciola italica luciferase in HEK-293 cells and in Escherichia coli strain BL21(DE3)pLysS
wild-type and mutant enzymes are expressed as GST-fusion proteins
wild-type and mutant enzymes expressed as glutathione-S-transferase-fusion proteins containing the additional N-terminal peptide Gly-Pro-Leu-Gly-SER, which remains after PreScission protease cleavage from GST, expression in Escherichia coli