1.13.12.6: Cypridina-luciferin 2-monooxygenase
This is an abbreviated version!
For detailed information about Cypridina-luciferin 2-monooxygenase, go to the full flat file.
Word Map on EC 1.13.12.6
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1.13.12.6
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bioluminescence
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luminescence
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hilgendorfii
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luciferases
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ostracod
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firefly
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analysis
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gaussia
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diagnostics
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molecular biology
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biotechnology
- 1.13.12.6
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bioluminescence
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luminescence
- hilgendorfii
- luciferases
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ostracod
- firefly
- analysis
- gaussia
- diagnostics
- molecular biology
- biotechnology
Reaction
Synonyms
Apogon luciferase, Apogon luciferase 1, CLase, CLuc, Cypridina luciferase, Cypridina luciferin 2-monooxygenase, Cypridina noctiluca luciferase, Cypridina-type luciferase, Fbp, FBP-IgG, luciferase (Cypridina luciferin), PGE2-luciferase, Vargula luciferase
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Natural Substrates Products on EC 1.13.12.6 - Cypridina-luciferin 2-monooxygenase
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REACTION DIAGRAM
Cypridina luciferin + O2
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fusion enzyme + 10 mM luciferin (dissolved in ethanol in 30 mM Tris-HCl (pH 7.6) with 10 mM EDTA) with ATP (250 mM), CoA (250 microM), and MgCl2 (5 mM) in 100 mM Tris-HCl, pH 7.8
Cypridina luciferase fusion enzyme expressed in Escherichia coli is soluble but does not exhibit bioluminescence
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Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
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Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
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bioluminescence
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Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
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bioluminescence, maximum wavelength emission at 465 nm
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Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
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conjugated enzyme exhibits bimodal bioluminescence spectrum at 460 nm and 675 nm, exhibits higher light intensity in mouse blood than unconjugated enzyme, far-red emission peak dominates in blood while the 460 nm peak dominates in buffers
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Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
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Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
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-
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?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
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-
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?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
-
-
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
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-
-
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
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-
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?