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1.13.11.9: 2,5-dihydroxypyridine 5,6-dioxygenase

This is an abbreviated version!
For detailed information about 2,5-dihydroxypyridine 5,6-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.9

Reaction

2,5-dihydroxypyridine
+
O2
=
N-formylmaleamic acid

Synonyms

2,5-DHP dioxygenase, 2,5-dihydroxypyridine dioxygenase, 2,5-dihydroxypyridine oxygenase, 2,5DHP dioxygenase, EC 1.13.1.9, NicX, NicX protein, oxygenase, 2,5-dihydroxypyridine 5,6-di-, pyridine-2,5-diol dioxygenase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.9 2,5-dihydroxypyridine 5,6-dioxygenase

Purification

Purification on EC 1.13.11.9 - 2,5-dihydroxypyridine 5,6-dioxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Escherichia coli cells overexpressing NicX are harvested and disrupted by passage through a French press, supernatant applied to DEAE-cellulose column with 50 mM NaH2PO4 buffer, pH 7.5, washed with buffer (0.1-0.3 M) containing 0.1 M NaCl, fraction with 2,5-dihydroxypyridine deoxygenase activity pooled and dialyzed with 20 mM NaH2PO4 buffer, pH 7.5, loaded onto hydroxyapatite column, eluted with 20-100 mM NaH2PO4 buffer, pH 7.0, fractions with enzyme activity pooled, dialyzed in 20 mM NaH2PO4 buffer, pH 7.5, and concentrated
-
recombinant enzyme
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange and hydroxyapatite chromatography
-