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1.13.11.60: linoleate 8R-lipoxygenase

This is an abbreviated version!
For detailed information about linoleate 8R-lipoxygenase, go to the full flat file.

Word Map on EC 1.13.11.60

Reaction

linoleate
+
O2
=
(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate

Synonyms

5,8-LDS, 5,8-linoleate diol synthase, 6,8-diol synthase, 6R,8R-linoleate diol synthase, 6R,8RLDS, 7,8-diol synthase, 7,8-LDS, 7,8-linoleate diol synthase, 8(R)-dioxygenase, 8,11-hydroperoxide isomerase, 8-DOX, 8R-dioxygenase, 8R-DOX, 8R-DOX-5,8-LDS, 8R-DOX-7,8-LDS, 8R-DOX-LDS, dioxygenase-cytochrome P450, DOX-CYP, EC 1.13.11.44, EGE82165, FOXB_09952, GLRG_10013, linoleate 7,8-LDS, linoleate 8,11-LDS, linoleate diol synthase, linoleic acid 8R-dioxygenase, ODH51007, orphan DOX-CYP, PpoA

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.60 linoleate 8R-lipoxygenase

Cloned

Cloned on EC 1.13.11.60 - linoleate 8R-lipoxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Star (DE3) cells
expressed in Escherichia ER2566 cells
-
expressed in Spodoptera frugiperda (Sf21) insect cells with native 8R-dioxygenase and hydroperoxide isomerase activities
expression in Escherichia coli
expression in Escherichia coli and in insect cells
expression in Pichia pastoris changes the position and stereospecificity of a hydroperoxide isomerase possibly due to N- or O-linked mannosides in the vicinity of the heme group, whereas the 8(R)-dioxygenase activity is identical with native 7,8-linoleate diol synthase
-
expression in SF21 cells
-
expression of wild-type and mutant enzymes in Escherichia coli strain BL21
gene GLRG_10013, phylogenetic analysis
phylogenetic tree, recombinant expression of wild-type and mutant His-tagged enzymes in Escherichia coli strain ER2566
recombinant expression of His-tagged enzyme in Escherichia coli strain ER2566