1.13.11.6: 3-hydroxyanthranilate 3,4-dioxygenase

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For detailed information about 3-hydroxyanthranilate 3,4-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.6

1.13.11.6

quinolinic

kynureninase

quin

2,3-dioxygenase

3-monooxygenase

excitotoxin

phosphoribosyltransferase

kynurenine-oxoglutarate

aminocarboxymuconate-semialdehyde

kynurenic

3-hydroxykynurenine

ibotenic

epilepsy-prone

picolinic

drug development

medicine

pharmacology

Reaction

Synonyms

3-HAD, 3-HAO, 3-hydroxyanthranilate 3,4-dioxygenase, 3-hydroxyanthranilate oxygenase, 3-hydroxyanthranilic acid oxidase, 3-hydroxyanthranilic acid oxygenase, 3-hydroxyanthranilic oxygenase, 3HAO, EC 1.13.1.6, HAD, HAO, oxygenase, 3-hydroxyanthranilate 3,4-di-

ECTree

1 Oxidoreductases

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.13.11 With incorporation of two atoms of oxygen

1.13.11.6 3-hydroxyanthranilate 3,4-dioxygenase

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Results	in table
1	Activating Compound
5583	AA Sequence
4	Application
1	CAS Registry Number
8	Cloned(Commentary)
1	Cofactor
6	Crystallization (Commentary)
24	Disease/ Diagnostics
8	General Information
2	General Stability
35	Inhibitors
8	Ki Value [mM]
18	KM Value [mM]
3	Localization
20	Metals/Ions
15	Molecular Weight [Da]
14	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
213	Organism
8	Pathway
39	PDB ID
6	pH Optimum
4	pH Range
2	pH Stability
2	pI Value
18	Protein Variants
13	Purification (Commentary)
4	Reaction
3	Reaction Type
36	Reference
20	Source Tissue
12	Specific Activity [micromol/min/mg]
8	Storage Stability
44	Substrates and Products (Substrate)
6	Subunits
22	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
2	Temperature Range [°C]
1	Temperature Stability [°C]
5	Turnover Number [1/s]

Purification

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Purification on EC 1.13.11.6 - 3-hydroxyanthranilate 3,4-dioxygenase

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5 entries

bovine kidney homogenized in 0.1 M potassium dihydrogen phosphate buffer with 20% glycerol and 3 mM 2-mercaptoethanol, pH 7.4, centrifuged, protein fraction of supernatant that precipitates from 34-62% saturated (NH4)2SO4 is resuspended in 5 mM potassium dihydrogen phosphate buffer with 20% glycerol and 3 mM 2-mercaptoethanol, pH 7.4, dialyzed against same buffer containing protease inhibitor PMSF (0.1 mM), applied to DEAE Sephadex A-50 column, fractions with enzyme activity pooled and concentrated by precipitation with 80%-saturated (NH4)2SO4, dialyzed against 10 mM Tris-HCl with 20% glycerol and 3 mM 2-mercaptoethanol, pH 7.4, applied to Blue-Sepharose CL-4B column, concentration of active fractions by ultrafiltration through YM-10 membrane

liver

pure liver enzyme, partielly purified brain enzyme

Rattus norvegicus

439367

recombinant

Saccharomyces cerevisiae

677020

recombinant His6-MBP-tagged enzyme from Escherichia coli by nickel affinity chromatography and cleavage of the His-tag by TEV protease, elimination of the tags by nickel affinity and amylose affinity chromatography, te final step is gel filtration

Homo sapiens

P46952

741516

succesive size exclusion and affinity columns

Bos taurus

658927