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1.13.11.54: acireductone dioxygenase [iron(II)-requiring]

This is an abbreviated version!
For detailed information about acireductone dioxygenase [iron(II)-requiring], go to the full flat file.

Word Map on EC 1.13.11.54

Reaction

1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one
+
O2
=
4-(methylsulfanyl)-2-oxobutanoate
 +
formate

Synonyms

1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase, aci-reductone dioxygenase, acireductone dioxygenase, acireductone dioxygenase 1, ADI1, ARD, ARD', ARD1, ARD4, ARDp, Fe(II)-bound acireductone dioxygenase, Fe-ARD, MTCBP-1, MtnD, OsARD1, Ymr009p

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.54 acireductone dioxygenase [iron(II)-requiring]

Cloned

Cloned on EC 1.13.11.54 - acireductone dioxygenase [iron(II)-requiring]

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 (DE3) codon Plus RIPL cells
expressed in Escherichia coli BL21(DE3) cells
A0A2V2XFM8; A0A2V2VIR7
expressed in Escherichia coli BL21(DE3)-CodonPlus-RIPL cells
expressed in Escherichia coli strain BL21(DE3)pLysS
expressed in Oryza sativa subsp. indica rice variety ASD16 through
-
expressed in Solanum lycopersicum cultivar Micro-Tom and in Nicotiana tabacum
expression in Escherichia coli
transient expression of GFP-acireductone dioxygenase 1 in Arabidopsis thaliana protoplasts. The coding regions of ARD1–4 are cloned into pDEST17TM and pDEST15TM containing an N-terminal poly-His tag and an N-terminal GST tag, respectively, and are expressed in Escherichia coli strain Rosetta BL21