1.13.11.49: chlorite O2-lyase
This is an abbreviated version!
For detailed information about chlorite O2-lyase, go to the full flat file.
Word Map on EC 1.13.11.49
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1.13.11.49
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perchlorate
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chlorate
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dechloromonas
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perchlorate-reducing
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dismutases
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clo2
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dismutation
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high-spin
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low-spin
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dechloratans
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defluvii
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chlorate-reducing
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nitrospira
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ideonella
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aromatica
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azospira
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hemqs
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environmental protection
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molecular biology
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biotechnology
- 1.13.11.49
- perchlorate
- chlorate
- dechloromonas
-
perchlorate-reducing
- dismutases
- clo2
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dismutation
-
high-spin
-
low-spin
- dechloratans
- defluvii
-
chlorate-reducing
- nitrospira
- ideonella
- aromatica
- azospira
-
hemqs
- environmental protection
- molecular biology
- biotechnology
Reaction
Synonyms
chlorite dismutase, CLD, Cyan7425_1434, dimutase, chlorite, HemQ, Pfam chlorite dismutase, PitA
ECTree
1.13.11.49 chlorite O2-lyaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 1.13.11.49 - chlorite O2-lyase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
chloride + O2
chlorite
-
chlorite dismutase catalyzes O2 release from chlorite with exquisite efficiency and specificity
-
-
r
chloride + O2
chlorite
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chlorite dismutase catalyzes O2 release from chlorite with exquisite efficiency and specificity, the protonation state of the heme environment influences dioxygen formation. The reaction proceeds via an acid-base transition with pKa of 8.7, spectroscopic properties, ligand binding affinities, and steady-state kinetics, unique structure-activity model, overview
-
-
r
chloride + O2
chlorite
chlorite dismutase is a unique heme enzyme which transforms chlorite to chloride and molecular oxygen
-
-
r
chloride + O2
chlorite
Arg173 plays a key role in (i) controlling of ligand and substrate access and binding and (ii) in chlorite dismutation reaction, mechanism, overview. The flexible residue modulates the electrostatic potential and size of the active site entrance and might be involved in keeping transiently formed hypochlorite in place for final molecular oxygen and chloride formation
-
-
r
chlorite
chloride + O2
-
-
reaction provides oxygen for aerobic benzene biodegradation
-
?
chlorite
chloride + O2
-
-
reaction provides oxygen for aerobic benzene biodegradation
-
?
chlorite
chloride + O2
catalytic mechanism via ferryl species and ClO--anion
-
-
?
chlorite
chloride + O2
the enzyme is highly specific for chlorite
-
-
?
chlorite
chloride + O2
catalytic mechanism via ferryl species and ClO--anion
-
-
?
chlorite
chloride + O2
-
-
highly specific (no 18O from 18O-labeled water), no other side products with 18O-labeled ClO2- detectable
-
?
chlorite
chloride + O2
-
the heme enzyme rapidly converts chlorite to chloride and O2, suggesting a simple approach to overcoming both the technical difficulties in the systematic variation of the O2 concentration and its modest solubility
-
-
?
chlorite
chloride + O2
Stutzerimonas chloritidismutans
-
-
degradation of n-alkane or intermediates + chlorate
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?
chlorite
chloride + O2
Stutzerimonas chloritidismutans
-
-
water is not involved in the O2-building reaction (no inclusion of 18O-isotope from labeled water)
-
?
chlorite
chloride + O2
Stutzerimonas chloritidismutans AW-1 / DSM 13592
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-
degradation of n-alkane or intermediates + chlorate
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?
chlorite
chloride + O2
Stutzerimonas chloritidismutans AW-1 / DSM 13592
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-
water is not involved in the O2-building reaction (no inclusion of 18O-isotope from labeled water)
-
?
chlorite
chloride + O2
Stutzerimonas chloritidismutans AW-1(T)
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-
degradation of n-alkane or intermediates + chlorate
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?
additional information
?
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the enzyme can reversibly bind acetate to form a green colored high spin ferric complex
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-
-
additional information
?
-
-
PitA possesses both chlorite dismutase-related (N-terminus) and antibiotic biosynthesis monooxygenase-related (C-terminus) activities
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-
?
additional information
?
-
-
PitA possesses both chlorite dismutase-related (N-terminus) and antibiotic biosynthesis monooxygenase-related (C-terminus) activities
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-
?
additional information
?
-
upon reduction of chlorite, hypochlorite is formed and kept in the reaction sphere for recombination with the oxoiron(IV) group of Compound I. Approximately one molecule of HOCl per 100 full cycles escapes and reacts with both the prosthetic group and the protein moiety, leading to irreversible inactivation of Cld is observed which is more pronounced with an increase in pH. HOCl traps like methionine can rescue the enzyme from inactivation
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-
?
additional information
?
-
chlorite binding to ferric Cld occurs spontaneously and residue Arg173 is important for recognition and to impair hypochlorite leakage from the reaction sphere
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-
?
additional information
?
-
-
chlorite binding to ferric Cld occurs spontaneously and residue Arg173 is important for recognition and to impair hypochlorite leakage from the reaction sphere
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-
?
additional information
?
-
-
although its sequence is highly similar to functional chlorite dismutases, the HemQ protein has no steady state reactivity with chlorite, very modest reactivity with H2O2 or peracetic acid, and no observable transient intermediates
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-
?
additional information
?
-
-
HemQ uses heme or porphyrin-like organic molecules as substrates. At pH 6.8, the enzyme exhibits relatively weak peroxidase activity with the reductant 2,2'-azino-bis[3-ethylbenzthiazoline-6-sulfonic acid], kinetics, overview
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-
?
additional information
?
-
-
although its sequence is highly similar to functional chlorite dismutases, the HemQ protein has no steady state reactivity with chlorite, very modest reactivity with H2O2 or peracetic acid, and no observable transient intermediates
-
-
?
additional information
?
-
-
HemQ uses heme or porphyrin-like organic molecules as substrates. At pH 6.8, the enzyme exhibits relatively weak peroxidase activity with the reductant 2,2'-azino-bis[3-ethylbenzthiazoline-6-sulfonic acid], kinetics, overview
-
-
?
additional information
additional information
-
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a diagnostic for peroxidase reactivity, 100 mM phosphate buffer (pH 6.8) and citrate phosphate buffer (pH 4.0)
no relevant peroxidase reactivity products, only in the presence of oxidant peroxyacetic acid peroxidase reactivity products are obtained (1.6 equivalents per mol oxidant)
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?
additional information
additional information
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-
guaiacol is a diagnostic for peroxidase reactivity, 100 mM phosphate buffer (pH 6.8) and citrate phosphate buffer (pH 4.0)
no relevant peroxidase reactivity products, only in the presence of oxidant peroxyacetic acid peroxidase reactivity products are obtained (1.6 equivalents per mol oxidant)
-
?
additional information
additional information
-
-
monochlorodimedone is a diagnostic for halogenation reactivity, 100 mM phosphate buffer (pH 6.8) and citrate phosphate buffer (pH 4.0)
no halogenation is observed, not even when Cl-, Br-, or I- are added in excess (10 mM) under the same conditions or when oxidants such as peroxyacetic acid or H2O2 are added
-
?
additional information
additional information
-
-
peroxyacetic acid with chlorite
in excess of peroxyacetic acid the formation of two high-valent ferryl intermediates can be observed with stopped-flow UV-vis spectrophotometry in 0.1 M sodium phosphate buffer, pH 7.0, 10°C and with electron paramagnetic resonance in 20% ethylene glycol, 80% 0.010 M phosphate buffer, pH 7.14, addition of substrate and enzyme at 4°C, flash freezing in liquid N2 for analysis
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?
additional information
additional information
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-
thioanisole is a diagnostic for peroxygenase reactivity, 100 mM phosphate buffer (pH 6.8) and citrate phosphate buffer (pH 4.0)
no relevant sulfoxide products that would point to peroxygenase reactivity, only in the presence of oxidant peroxyacetic acid sulfoxide formation is observed (0.247 sulfoxide per mol oxidant)
-
?
additional information
additional information
-
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a diagnostic for peroxidase reactivity, 100 mM phosphate buffer (pH 6.8) and citrate phosphate buffer (pH 4.0)
no relevant peroxidase reactivity products, only in the presence of oxidant peroxyacetic acid peroxidase reactivity products are obtained (1.6 equivalents per mol oxidant)
-
?
additional information
additional information
-
-
guaiacol is a diagnostic for peroxidase reactivity, 100 mM phosphate buffer (pH 6.8) and citrate phosphate buffer (pH 4.0)
no relevant peroxidase reactivity products, only in the presence of oxidant peroxyacetic acid peroxidase reactivity products are obtained (1.6 equivalents per mol oxidant)
-
?
additional information
additional information
-
-
monochlorodimedone is a diagnostic for halogenation reactivity, 100 mM phosphate buffer (pH 6.8) and citrate phosphate buffer (pH 4.0)
no halogenation is observed, not even when Cl-, Br-, or I- are added in excess (10 mM) under the same conditions or when oxidants such as peroxyacetic acid or H2O2 are added
-
?
additional information
additional information
-
-
peroxyacetic acid with chlorite
in excess of peroxyacetic acid the formation of two high-valent ferryl intermediates can be observed with stopped-flow UV-vis spectrophotometry in 0.1 M sodium phosphate buffer, pH 7.0, 10°C and with electron paramagnetic resonance in 20% ethylene glycol, 80% 0.010 M phosphate buffer, pH 7.14, addition of substrate and enzyme at 4°C, flash freezing in liquid N2 for analysis
-
?
additional information
additional information
-
-
thioanisole is a diagnostic for peroxygenase reactivity, 100 mM phosphate buffer (pH 6.8) and citrate phosphate buffer (pH 4.0)
no relevant sulfoxide products that would point to peroxygenase reactivity, only in the presence of oxidant peroxyacetic acid sulfoxide formation is observed (0.247 sulfoxide per mol oxidant)
-
?
