1.13.11.49: chlorite O2-lyase
This is an abbreviated version!
For detailed information about chlorite O2-lyase, go to the full flat file.
Word Map on EC 1.13.11.49
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1.13.11.49
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perchlorate
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chlorate
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dechloromonas
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perchlorate-reducing
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dismutases
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clo2
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dismutation
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high-spin
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low-spin
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dechloratans
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defluvii
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chlorate-reducing
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nitrospira
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ideonella
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aromatica
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azospira
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hemqs
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environmental protection
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molecular biology
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biotechnology
- 1.13.11.49
- perchlorate
- chlorate
- dechloromonas
-
perchlorate-reducing
- dismutases
- clo2
-
dismutation
-
high-spin
-
low-spin
- dechloratans
- defluvii
-
chlorate-reducing
- nitrospira
- ideonella
- aromatica
- azospira
-
hemqs
- environmental protection
- molecular biology
- biotechnology
Reaction
Synonyms
chlorite dismutase, CLD, Cyan7425_1434, dimutase, chlorite, HemQ, Pfam chlorite dismutase, PitA
ECTree
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Purification
Purification on EC 1.13.11.49 - chlorite O2-lyase
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anion exchange column chromatography and S-200 HR gel filtration
cell pellet suspended in 0.05 M Tris-HCl buffer, pH 9.5 and 0.05 EDTA for 30 min at room temperature, centrifuged, supernatant ultracentrifuged, red supernatant containing periplasmic fraction separated from pellet, diluted with 10 mM Tris-HCl buffer, pH 7.2, purification with ÄKTAfplc, diluted supernatant loaded onto hydroxyapatite column with 10 mM Tris-HCl, pH 7.2, enzyme elutes at the start of a linear gradient of 10 mM Tris-Hcl, pH 7.2 to 450 mM potassium phosphate, pH 7.2, storage at -20°C
Stutzerimonas chloritidismutans
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cell-free extracts are prepared from strain AW-1 cells grown in anaerobic medium with n-decane as sole carbon and energy source and chlorate as electron acceptor, centrifugation, storage of extracts under N2 gas at 4°C
Stutzerimonas chloritidismutans
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centrifuged cell extract, cells grown in AW-1-sulfate medium with benzene and chlorate or benzene and oxygen
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HiLoad 16/60 Superdex 200 prep-grade column, equilibrated with 20 mM Tris-HCl (pH 7.5) and 135 mM NaCl to determin molecular Stokes radius
His-trap affinity column chromatography and Superdex 200 gel filtration
recombinant enzyme from Escherichia coli by anion exchange chromatography and gel filtration
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recombinant His-tagged enzyme from Escherichia coli strain Tuner (DE3) by affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain Tuner (DE3) by nickel affinity chromatography and gel filtration
recombinant mature Cld from Tuner(DE3) Escherichia coli cells by anion exchange chromatography and gel filtration
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recombinant N-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant Strep-tagged enzyme from Escherichia coli strain Tuner (DE3) by affinity chromatography, the Strep-II-tag is fully cleaved off using TEV-protease, followed by gel filtration
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the EPR spectroscopic signature of the as-purified Cld samples is affected by the buffer composition. Potassium phosphate buffer is the only buffer that affects neither the spectral nor the kinetic properties of Cld