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1.13.11.49: chlorite O2-lyase

This is an abbreviated version!
For detailed information about chlorite O2-lyase, go to the full flat file.

Word Map on EC 1.13.11.49

Reaction

chloride
+
O2
=
Chlorite

Synonyms

chlorite dismutase, CLD, Cyan7425_1434, dimutase, chlorite, HemQ, Pfam chlorite dismutase, PitA

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.49 chlorite O2-lyase

Cloned

Cloned on EC 1.13.11.49 - chlorite O2-lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain Tuner (DE3)
enzyme gene chemically synthesized without signal sequence, cloned into vector pET28a with histidine tag and thrombin cleavage site (removed before crystallization), and overexpressed
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 Gold (DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) pLysS cells
-
expressed in Escherichia coli Tuner (DE3) cells
-
expressed in Escherichia coli Tuner(DE3) cells
-
expression in Escherichia coli
expression in Escherichia coli BL21
-
expression in Escherichia coli results in an active enzyme with similar spectral properties as the native enzyme
-
expression in Escherichia coli, EPR differences between the native and recombinant enzymes as well as the spectrophotometric titration of the native enzyme
-
expression of C-terminally His-tagged enzyme in Escherichia coli strain Tuner (DE3)
expresssion in Escherichia coli
gene hemQ, overexpression in Escherichia coli, complementation of the hemQ-defective Staphylococcus aureus mutant
-
phylogenetic analysis, expression of N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
recombinant expression of the enzyme without the N-terminal signal, but with N-terminal TEV-cleavable Strep-II tag in Escherichia coli strain Tuner (DE3)
-
the gene encoding mature Cld, secretion peptide removed and replaced with an N-terminal Met residue, is expressed in untagged form from the pET41a vector in Tuner(DE3) Escherichia coli cells
-