1.13.11.39: biphenyl-2,3-diol 1,2-dioxygenase
This is an abbreviated version!
For detailed information about biphenyl-2,3-diol 1,2-dioxygenase, go to the full flat file.
Reaction
+
=
Synonyms
2'-aminobiphenyl-2,3-diol 1,2-dioxygenase, 2,3-DBDO, 2,3-DBPD, 2,3-DHBD, 2,3-DHBP dioxygenase, 2,3-dihydroxy-biphenyl 1,2-dioxygenase, 2,3-dihydroxybiphenyl 1,2-dioxygenase, 2,3-dihydroxybiphenyl dioxygenase, 2,3-dihydroxybiphenyl-1,2-dioxygenase, 2,3-dihydroxybiphenyl:oxygen 1,2 oxidoreductase, 2,3-diOH-biphenyl 1,2-dioxygenase, 2,3DHBD, 2-aminobiphenyl-2,3-diol-1,2-dioxygenase, 23 OHBP oxygenase, 3-phenylcatechol dioxygenase, biphenyl-2,3-diol dioxygenase, BPDO, BphC, BphC1, BphC1-BN6, BphC2, BphC2-BN6, BphC3, bphC5, BphCLA-4, BphC_JF8, BphC_LA-4, C23o, CarB, CarBaBb, DHB12O, DHBD, EtbC, manganese(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase, RrbphCI
ECTree
KM Value
KM Value on EC 1.13.11.39 - biphenyl-2,3-diol 1,2-dioxygenase
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0.014
2'-aminobiphenyl-2,3-diol
-
pH 7.5, 25°C
0.001 - 0.019
2'-chloro-2,3-dihydroxybiphenyl
0.0026
2,2'-dichlorobiphenyl
-
-
0.000095 - 0.74
2,3-Dihydroxybiphenyl
0.00045 - 0.01
3'-chloro-2,3-dihydroxybiphenyl
0.0033
3,3'-dichlorobiphenyl
-
-
0.007 - 0.03
3,5-dichlorocatechol
0.025 - 2.1
3-chlorocatechol
0.0108
3-formylcatechol
-
in 50 mM Hepes (pH 7.5) at 25°C
0.001 - 14.6
3-methylcatechol
0.0015 - 0.012
4'-chloro-2,3-dihydroxybiphenyl
0.025 - 0.0496
4-Chlorocatechol
0.0273 - 93
4-methylcatechol
0.00046 - 0.087
biphenyl-2,3-diol
additional information
additional information
-
0.001
2'-chloro-2,3-dihydroxybiphenyl
-
25°C, pH 7.5
0.018
2'-chloro-2,3-dihydroxybiphenyl
-
25°C, pH 7.5
0.019
2'-chloro-2,3-dihydroxybiphenyl
-
25°C, pH 7.5
0.000095
2,3-Dihydroxybiphenyl
60°C, pH 7.5
0.0003
2,3-Dihydroxybiphenyl
60°C
0.001
2,3-Dihydroxybiphenyl
25°C
0.001
2,3-Dihydroxybiphenyl
-
25°C, pH 7.5
0.003
2,3-Dihydroxybiphenyl
-
in presence of 3-chlorocatechol
0.007
2,3-Dihydroxybiphenyl
-
-
0.0125
2,3-Dihydroxybiphenyl
strain OU83
0.014
2,3-Dihydroxybiphenyl
strain OU83
0.0145
2,3-Dihydroxybiphenyl
strain OU83
0.0189
2,3-Dihydroxybiphenyl
pH 8.0, 20°C
0.022
2,3-Dihydroxybiphenyl
-
-
0.026
2,3-Dihydroxybiphenyl
-
25°C, pH 7.5
0.029
2,3-Dihydroxybiphenyl
-
25°C, pH 7.5
0.032
2,3-Dihydroxybiphenyl
-
Glu79His mutant
0.06
2,3-Dihydroxybiphenyl
25°C
0.74
2,3-Dihydroxybiphenyl
-
-
0.00045
3'-chloro-2,3-dihydroxybiphenyl
-
25°C, pH 7.5
0.01
3'-chloro-2,3-dihydroxybiphenyl
-
25°C, pH 7.5
0.007
3,5-dichlorocatechol
-
Glu79His mutant
0.03
3,5-dichlorocatechol
-
-
0.025
3-chlorocatechol
-
Glu79His mutant
0.8
3-chlorocatechol
60°C
1.6
3-chlorocatechol
25°C
2.1
3-chlorocatechol
25°C
0.001
3-methylcatechol
60°C, pH 7.5
0.0021
3-methylcatechol
pH 7.5, 30°C
0.024
3-methylcatechol
pH 9.0, 25°C, recombinant His-tagged enzyme
0.0616
3-methylcatechol
pH 7.5, 30°C
0.0688
3-methylcatechol
pH 8.0, 20°C
0.097
3-methylcatechol
-
Glu79His mutant
0.1
3-methylcatechol
-
25°C, pH 7.5
0.1306
3-methylcatechol
pH 7.5, 30°C
0.18
3-methylcatechol
-
-
0.19
3-methylcatechol
-
-
0.49
3-methylcatechol
pH 9.0, 40°C, recombinant enzyme
1.1
3-methylcatechol
60°C
1.23
3-methylcatechol
-
25°C, pH 7.5
1.94
3-methylcatechol
-
25°C, pH 7.5
2.1
3-methylcatechol
25°C
14.6
3-methylcatechol
25°C
0.0015
4'-chloro-2,3-dihydroxybiphenyl
-
25°C, pH 7.5
0.011
4'-chloro-2,3-dihydroxybiphenyl
-
25°C, pH 7.5
0.012
4'-chloro-2,3-dihydroxybiphenyl
-
25°C, pH 7.5
0.025
4-Chlorocatechol
60°C, pH 7.5
0.0496
4-Chlorocatechol
pH 8.0, 20°C
0.0273
4-methylcatechol
pH 7.5, 30°C
0.11
4-methylcatechol
60°C, pH 7.5
0.2824
4-methylcatechol
pH 7.5, 30°C
0.3597
4-methylcatechol
pH 8.0, 20°C
0.3605
4-methylcatechol
pH 7.5, 30°C
2.8
4-methylcatechol
-
25°C, pH 7.5
5.6
4-methylcatechol
25°C
7.6
4-methylcatechol
60°C
61
4-methylcatechol
-
25°C, pH 7.5
93
4-methylcatechol
-
25°C, pH 7.5
0.00046
biphenyl-2,3-diol
-
in 50 mM Hepes (pH 7.5) at 25°C
0.0015
biphenyl-2,3-diol
-
pH not specified in the publication, temperature not specified in the publication
0.00213
biphenyl-2,3-diol
pH 7.5, 30°C
0.00364
biphenyl-2,3-diol
-
pH 8.0, 30°C, free enzyme
0.0049
biphenyl-2,3-diol
pH 7.5, 30°C
0.0067
biphenyl-2,3-diol
pH 9.0, 40°C, recombinant enzyme
0.01022
biphenyl-2,3-diol
-
pH 8.0, 30°C, immobilized enzyme
0.0117
biphenyl-2,3-diol
pH 9.0, 25°C, recombinant His-tagged enzyme
0.0391
biphenyl-2,3-diol
pH 7.5, 30°C
0.087
biphenyl-2,3-diol
-
pH 9.0, 25°C
0.0062
catechol
pH 7.5, 30°C
0.01672
catechol
pH 7.5, 30°C
0.0589
catechol
-
in 50 mM Hepes (pH 7.5) at 25°C
0.0976
catechol
pH 7.5, 30°C
0.1
catechol
60°C, pH 7.5
0.281
catechol
pH 8.0, 20°C
0.606
catechol
pH 9.0, 40°C, recombinant enzyme
0.625
catechol
pH 9.0, 25°C, recombinant His-tagged enzyme
0.67
catechol
-
Glu79His mutant
0.67
catechol
-
mutant enzyme E79H, at pH 7.5, temperature not specified in the publication
1.13
catechol
-
wild type enzyme, at pH 7.5, temperature not specified in the publication
2.4
catechol
-
25°C, pH 7.5
0.028
O2
-
biphenyl as substrate
0.19
O2
-
3,3'-dichlorobiphenyl as substrate
0.75
O2
-
2,2'-dichlorobiphenyl as substrate
additional information
additional information
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Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten constants derived from LineweaverBurk plots
-