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1.13.11.38: 1-hydroxy-2-naphthoate 1,2-dioxygenase

This is an abbreviated version!
For detailed information about 1-hydroxy-2-naphthoate 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.38

Reaction

1-hydroxy-2-naphthoate
+
O2
=
(3Z)-4-(2-carboxyphenyl)-2-oxobut-3-enoate

Synonyms

1-H2NA dioxygenase, 1-HNDO, 1-hydroxy-2-naphthoate 1,2-dioxygenase, 1-hydroxy-2-naphthoate dioxygenase, 1-hydroxy-2-naphthoate-degrading enzyme, 1-hydroxy-2-naphthoic acid dioxygenase, 1H2NDO, Diox1, Diox2, oxygenase, 1-hydroxy-2-naphthoate di-, Sphe3 1-H2NA dioxygenase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.38 1-hydroxy-2-naphthoate 1,2-dioxygenase

Crystallization

Crystallization on EC 1.13.11.38 - 1-hydroxy-2-naphthoate 1,2-dioxygenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
mutant A85H and W104Y variants and mutant W104Y with gentiate, sitting drop vapor diffusion method, 4°C, mixing of 0.001 ml of protein solution with 0.0008 ml of crystallization solution containing 8% PEG10000 and 0.1 M Tris-HCl pH 8.0, with 0.0002 ml of a 0.1 M calcium chloride solution, crystals quality is also improved using seeding techniques, X-ray diffraction structure determination and analysis at 2.5-2.7 A resolution
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