1.13.11.34: arachidonate 5-lipoxygenase
This is an abbreviated version!
For detailed information about arachidonate 5-lipoxygenase, go to the full flat file.
Word Map on EC 1.13.11.34
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1.13.11.34
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cyclooxygenase
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prostaglandin
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leukocyte
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neutrophil
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eicosanoids
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cox-2
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asthma
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indomethacin
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ionophore
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phospholipase
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zileuton
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polymorphonuclear
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thromboxane
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flap
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airway
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peritoneal
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5-hete
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platelet
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eosinophil
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mast
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cysteinyl
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allergic
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edema
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12-lipoxygenase
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histamine
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lta4
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nordihydroguaiaretic
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lipoxygenases
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paf
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ndga
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bronchoconstriction
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5-hydroxyeicosatetraenoic
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asthmatic
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basophil
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platelet-activating
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anaphylaxis
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zymosan
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antigen-induced
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lipoxins
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montelukast
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hetes
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mpges-1
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carrageenan-induced
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a23187-stimulated
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paf-induced
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pro-resolving
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synthesis
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bronchospasm
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medicine
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drug development
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14carachidonic
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pharmacology
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bronchoconstrictors
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ulcerogenic
- 1.13.11.34
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cyclooxygenase
- prostaglandin
- leukocyte
- neutrophil
-
eicosanoids
- cox-2
- asthma
- indomethacin
-
ionophore
- phospholipase
- zileuton
-
polymorphonuclear
-
thromboxane
- flap
- airway
- peritoneal
-
5-hete
- platelet
-
eosinophil
-
mast
-
cysteinyl
-
allergic
- edema
-
12-lipoxygenase
- histamine
- lta4
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nordihydroguaiaretic
- lipoxygenases
- paf
- ndga
-
bronchoconstriction
-
5-hydroxyeicosatetraenoic
-
asthmatic
-
basophil
-
platelet-activating
- anaphylaxis
- zymosan
-
antigen-induced
-
lipoxins
- montelukast
-
hetes
- mpges-1
-
carrageenan-induced
-
a23187-stimulated
-
paf-induced
-
pro-resolving
- synthesis
- bronchospasm
- medicine
- drug development
-
14carachidonic
- pharmacology
-
bronchoconstrictors
-
ulcerogenic
Reaction
Synonyms
5-lipoxygenase, 5-LO, 5-LO1, 5-LOX, 5DELTA-lipoxygenase, 5LO, 5LOX-1, ALOX5, arachidonate 5-LO, arachidonate:oxygen oxidoreductase, arachidonic 5-lipoxygenase, arachidonic acid 5-lipoxygenase, C-5-lipoxygenase, DELTA5-lipoxygenase, H5-LO, leukotriene A4 synthase, leukotriene-A4 synthase, lipoxygenase 15, lipoxygenase 5, lipoxygenase-1, LO-1, LOX-15, LOX-5, LTA synthase, LTA4 synthase, oxygenase, arachidonate, 5-lip-, PMNL 5-lipoxygenase
ECTree
1.13.11.34 arachidonate 5-lipoxygenaseAdvanced search results
results (
results (KM Value
KM Value on EC 1.13.11.34 - arachidonate 5-lipoxygenase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.014
(5S,6E,8Z,11Z,14Z)-5-hydroperoxyicosa-6,8,11,14-tetraenoate
pH 7.5, 21°C, recombinant enzyme
0.019
(5S,6E,8Z,11Z,14Z)-5-hydroperoxyicosa-6,8,11,14-tetraenoate
pH 7.5, 21°C, recombinant enzyme, with 0.2 mM ATP
0.01144
7,7-d2-arachidonate
pH 7.5, 25°C, UV-Vis spectrophotometric analysis
0.0051
arachidonate
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reaction mixture contains 1 mM DTT, 1 mM ATP, 2 mM CaCl2, and 0.01 mM indomethacin in phosphate buffered saline, at 37°C
0.02
arachidonate
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wild-type, pH and temperature not specified in the publication
0.06
arachidonate
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mutant DELTA1-114, pH and temperature not specified in the publication
additional information
additional information
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kinetics with lysophospholipid substrates
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additional information
additional information
Michaelis-Menten steady-state kinetics, kinetic isotope effect of 5-LOX, detailed overview. The reaction of 5-LOX with unlabeled arachidonate generates products from hydrogen abstraction at C7 (95% 5-HETE) with minor products derived from abstraction at C10 (5% 8-HETE). With 7,7-d2-arachidonate the selectivity remains skewed towards C7 hydrogen abstraction (59%) compared to C10 hydrogen abstraction products (41% 8-HETE), but the selectivity is significantly reduced compared to unlabeled arachidonate. 5-LOX exhibits isotope sensitive branching, shift in regiospecificity induced by deuterium-labeling. Dependence of the steady-state velocity on arachidonate concentration follows Michaelis-Menten kinetics up to 0.04-0.05 mM, effects of temperature and viscosity
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additional information
additional information
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Michaelis-Menten steady-state kinetics, kinetic isotope effect of 5-LOX, detailed overview. The reaction of 5-LOX with unlabeled arachidonate generates products from hydrogen abstraction at C7 (95% 5-HETE) with minor products derived from abstraction at C10 (5% 8-HETE). With 7,7-d2-arachidonate the selectivity remains skewed towards C7 hydrogen abstraction (59%) compared to C10 hydrogen abstraction products (41% 8-HETE), but the selectivity is significantly reduced compared to unlabeled arachidonate. 5-LOX exhibits isotope sensitive branching, shift in regiospecificity induced by deuterium-labeling. Dependence of the steady-state velocity on arachidonate concentration follows Michaelis-Menten kinetics up to 0.04-0.05 mM, effects of temperature and viscosity
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additional information
additional information
steady-state kinetics and kinetic mechanism, overview. Kinetic parameters for ATP-induced activation of 5-LOX
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additional information
additional information
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steady-state kinetics and kinetic mechanism, overview. Kinetic parameters for ATP-induced activation of 5-LOX
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